Partial isolation and some properties of enterotoxin produced by Bacillus cereus strains

Partial isolation and some properties of enterotoxin produced by Bacillus cereus strains

Extracellular proteins produced by Bacillus cereus AL-42 and AL-15 were fractioned by chromatography on QAE-Sephadex and Sephadex G75. This last chromatographic process resulted in three peaks. The major peak showed vascular permeability activity to rabbits, lethality to mice, and cytotoxicity to Ve...

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Main Authors: T. V. Guaycurus, A. C. Vicente, S. Giovanni De Simone, L. Rabinovitch
Format: Article
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 1993-03-01
Series:Memórias do Instituto Oswaldo Cruz.
Subjects:
Bacilus cereus
enterotoxin
toxic fator
purification
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761993000100020
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spelling doaj-37a1ae17148d410fa52accf6858ad4e92020-11-24T20:44:54ZengInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz.0074-02761678-80601993-03-0188113113410.1590/S0074-02761993000100020Partial isolation and some properties of enterotoxin produced by Bacillus cereus strainsT. V. GuaycurusA. C. VicenteS. Giovanni De SimoneL. RabinovitchExtracellular proteins produced by Bacillus cereus AL-42 and AL-15 were fractioned by chromatography on QAE-Sephadex and Sephadex G75. This last chromatographic process resulted in three peaks. The major peak showed vascular permeability activity to rabbits, lethality to mice, and cytotoxicity to Vero and Hela cells. The analysis by SDS-PAGE after ultrafiltration confirm recent findings that the enterotoxin is a compound with molecular mass > 30.000.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761993000100020Bacilus cereusenterotoxintoxic fatorpurification
collection DOAJ
language English
format Article
sources DOAJ
author T. V. Guaycurus
A. C. Vicente
S. Giovanni De Simone
L. Rabinovitch
spellingShingle T. V. Guaycurus
A. C. Vicente
S. Giovanni De Simone
L. Rabinovitch
Partial isolation and some properties of enterotoxin produced by Bacillus cereus strains
Memórias do Instituto Oswaldo Cruz.
Bacilus cereus
enterotoxin
toxic fator
purification
author_facet T. V. Guaycurus
A. C. Vicente
S. Giovanni De Simone
L. Rabinovitch
author_sort T. V. Guaycurus
title Partial isolation and some properties of enterotoxin produced by Bacillus cereus strains
title_short Partial isolation and some properties of enterotoxin produced by Bacillus cereus strains
title_full Partial isolation and some properties of enterotoxin produced by Bacillus cereus strains
title_fullStr Partial isolation and some properties of enterotoxin produced by Bacillus cereus strains
title_full_unstemmed Partial isolation and some properties of enterotoxin produced by Bacillus cereus strains
title_sort partial isolation and some properties of enterotoxin produced by bacillus cereus strains
publisher Instituto Oswaldo Cruz, Ministério da Saúde
series Memórias do Instituto Oswaldo Cruz.
issn 0074-0276
1678-8060
publishDate 1993-03-01
description Extracellular proteins produced by Bacillus cereus AL-42 and AL-15 were fractioned by chromatography on QAE-Sephadex and Sephadex G75. This last chromatographic process resulted in three peaks. The major peak showed vascular permeability activity to rabbits, lethality to mice, and cytotoxicity to Vero and Hela cells. The analysis by SDS-PAGE after ultrafiltration confirm recent findings that the enterotoxin is a compound with molecular mass > 30.000.
topic Bacilus cereus
enterotoxin
toxic fator
purification
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761993000100020
work_keys_str_mv AT tvguaycurus partialisolationandsomepropertiesofenterotoxinproducedbybacilluscereusstrains
AT acvicente partialisolationandsomepropertiesofenterotoxinproducedbybacilluscereusstrains
AT sgiovannidesimone partialisolationandsomepropertiesofenterotoxinproducedbybacilluscereusstrains
AT lrabinovitch partialisolationandsomepropertiesofenterotoxinproducedbybacilluscereusstrains
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