Partial isolation and some properties of enterotoxin produced by Bacillus cereus strains

Partial isolation and some properties of enterotoxin produced by Bacillus cereus strains

Extracellular proteins produced by Bacillus cereus AL-42 and AL-15 were fractioned by chromatography on QAE-Sephadex and Sephadex G75. This last chromatographic process resulted in three peaks. The major peak showed vascular permeability activity to rabbits, lethality to mice, and cytotoxicity to Ve...

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Bibliographic Details
Main Authors: T. V. Guaycurus, A. C. Vicente, S. Giovanni De Simone, L. Rabinovitch
Format: Article
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 1993-03-01
Series:Memórias do Instituto Oswaldo Cruz.
Subjects:
Bacilus cereus
enterotoxin
toxic fator
purification
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761993000100020
Description
Summary:Extracellular proteins produced by Bacillus cereus AL-42 and AL-15 were fractioned by chromatography on QAE-Sephadex and Sephadex G75. This last chromatographic process resulted in three peaks. The major peak showed vascular permeability activity to rabbits, lethality to mice, and cytotoxicity to Vero and Hela cells. The analysis by SDS-PAGE after ultrafiltration confirm recent findings that the enterotoxin is a compound with molecular mass > 30.000.
ISSN:0074-0276
1678-8060

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