Phage-displayed nanobody based double antibody sandwich chemiluminescent immunoassay for the detection of Cry2A toxin in cereals

Phage-displayed nanobody based double antibody sandwich chemiluminescent immunoassay for the detection of Cry2A toxin in cereals

In this study, we developed a double antibody sandwich chemiluminescent immunoassay (DAS-CLIA) based on phage-displayed nanobodies for the determination of Cry2A toxin. Nine phage-displayed nanobodies specifically to Cry2A were obtained from a naive nanobody library after four rounds of biopanning....

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Bibliographic Details
Main Authors: Yulou Qiu, Pan Li, Beibei Liu, Yuan Liu, Yulong Wang, Tingting Tao, Junli Xu, Bruce D. Hammock, Xianjin Liu, Rongfa Guan, Cunzheng Zhang
Format: Article
Language:English
Published: Taylor & Francis Group 2019-01-01
Series:Food and Agricultural Immunology
Subjects:
cry2a toxin
phage-displayed nanobody
chemiluminescence
sandwich immunoassay
Online Access:http://dx.doi.org/10.1080/09540105.2019.1642307

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