Identification of human Norovirus (HNoV) in domestic pig stool samples

• Main Authors: María F. Gutiérrez, Jazmín López, Andrea Ruíz, Cesar Osorio, Juan C. Ulloa
• Format: Article
• Language: English
• Published: Pontificia Universidad Javeriana 2011-08-01
• Series: Universitas Scientiarum
• Subjects: Human Norovirus; domestic pigs; diarrhea; zoonotic transmission; reservoirs
• Online Access: http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1648/1071

To determine the presence of NoVs as a possible causal zoonotic agent of acute diarrhea in pigs and humans. Materialsand methods. We collected a total of 77 samples from diarrheal children under 5 years and pigs under 2 months from La Chambatown in Tolima, Colombia. These samples were transported to the Laboratory of Virology of the Pontificia Universidad Javerianain Bogotá, and extraction with Trizol-reagent was done following the manufacturer’s instructions. After obtaining the RNA, thenext step was to perform RT-PCR for obtaining the expected amplification product of 213- bp NoVs. Finally, the positive samplesobtained in the RT-PCR were sequenced and analyzed by bioinformatics methods. Results. Six positive diarrheic samples fromchildren and a positive diarrheic sample from pigs were detected by a band of 231 bp. Five of the six positive samples in childrenand the positive pig sample were sequenced and analyzed. Conclusion. Given the close genetic relationship between pig andhuman sequences, this could be an indication of the potential existence of a common animal acting as a reservoir for human orother animal strains.