Brain Tissue Oxygen: In Vivo Monitoring with Carbon Paste Electrodes

In this communication we review selected experiments involving the use ofcarbon paste electrodes (CPEs) to monitor and measure brain tissue O2 levels in awakefreely-moving animals. Simultaneous measurements of rCBF were performed using the H2clearance technique. Voltammetric techniques used include...

Full description

Bibliographic Details
Main Authors: John P. Lowry, Fiachra B. Bolger
Format: Article
Language:English
Published: MDPI AG 2005-11-01
Series:Sensors
Subjects:
Online Access:http://www.mdpi.com/1424-8220/5/11/473/
id doaj-3738b7e0d0dc40358ceb979d2e3d6141
record_format Article
spelling doaj-3738b7e0d0dc40358ceb979d2e3d61412020-11-25T01:49:16ZengMDPI AGSensors1424-82202005-11-0151147348710.3390/s5110473Brain Tissue Oxygen: In Vivo Monitoring with Carbon Paste ElectrodesJohn P. LowryFiachra B. BolgerIn this communication we review selected experiments involving the use ofcarbon paste electrodes (CPEs) to monitor and measure brain tissue O2 levels in awakefreely-moving animals. Simultaneous measurements of rCBF were performed using the H2clearance technique. Voltammetric techniques used include both differential pulse (O2) andconstant potential amperometry (rCBF). Mild hypoxia and hyperoxia produced rapidchanges (decrease and increase respectively) in the in vivo O2 signal. Neuronal activation(tail pinch and stimulated grooming) produced similar increases in both O2 and rCBFindicating that CPE O2 currents provide an index of increases in rCBF when such increasesexceed O2 utilization. Saline injection produced a transient increase in the O2 signal whilechloral hydrate produced slower more long-lasting changes that accompanied the behavioralchanges associated with anaesthesia. Acetazolamide increased O2 levels through an increasein rCBF.http://www.mdpi.com/1424-8220/5/11/473/In-vivo voltammetrycerebral blood flowhypoxia and hyperoxianeuronal activationchloral hydrate anaesthesiaacetazolamide
collection DOAJ
language English
format Article
sources DOAJ
author John P. Lowry
Fiachra B. Bolger
spellingShingle John P. Lowry
Fiachra B. Bolger
Brain Tissue Oxygen: In Vivo Monitoring with Carbon Paste Electrodes
Sensors
In-vivo voltammetry
cerebral blood flow
hypoxia and hyperoxia
neuronal activation
chloral hydrate anaesthesia
acetazolamide
author_facet John P. Lowry
Fiachra B. Bolger
author_sort John P. Lowry
title Brain Tissue Oxygen: In Vivo Monitoring with Carbon Paste Electrodes
title_short Brain Tissue Oxygen: In Vivo Monitoring with Carbon Paste Electrodes
title_full Brain Tissue Oxygen: In Vivo Monitoring with Carbon Paste Electrodes
title_fullStr Brain Tissue Oxygen: In Vivo Monitoring with Carbon Paste Electrodes
title_full_unstemmed Brain Tissue Oxygen: In Vivo Monitoring with Carbon Paste Electrodes
title_sort brain tissue oxygen: in vivo monitoring with carbon paste electrodes
publisher MDPI AG
series Sensors
issn 1424-8220
publishDate 2005-11-01
description In this communication we review selected experiments involving the use ofcarbon paste electrodes (CPEs) to monitor and measure brain tissue O2 levels in awakefreely-moving animals. Simultaneous measurements of rCBF were performed using the H2clearance technique. Voltammetric techniques used include both differential pulse (O2) andconstant potential amperometry (rCBF). Mild hypoxia and hyperoxia produced rapidchanges (decrease and increase respectively) in the in vivo O2 signal. Neuronal activation(tail pinch and stimulated grooming) produced similar increases in both O2 and rCBFindicating that CPE O2 currents provide an index of increases in rCBF when such increasesexceed O2 utilization. Saline injection produced a transient increase in the O2 signal whilechloral hydrate produced slower more long-lasting changes that accompanied the behavioralchanges associated with anaesthesia. Acetazolamide increased O2 levels through an increasein rCBF.
topic In-vivo voltammetry
cerebral blood flow
hypoxia and hyperoxia
neuronal activation
chloral hydrate anaesthesia
acetazolamide
url http://www.mdpi.com/1424-8220/5/11/473/
work_keys_str_mv AT johnplowry braintissueoxygeninvivomonitoringwithcarbonpasteelectrodes
AT fiachrabbolger braintissueoxygeninvivomonitoringwithcarbonpasteelectrodes
_version_ 1725007615061131264