Inhibiting Effect and Its Mechanism of Ibandronate on the Proliferation of Humanized NSCLC A549 Cells in Vitro

• Main Authors: YAO Qiang, HUA Dong
• Format: Article
• Language: English
• Published: Third Party Medicine International Publishing Group Co. Limited 2014-09-01
• Series: Journal of International Translational Medicine
• Subjects: Lung cancer; A549 cell line; Ibandronate; hTERT
• Online Access: http://www.jitm.hk/EN/10.11910/2227-6394.2014.02.03.04
• ISSN: 2227-6394; 2227-6394

Objective: To explore the effect of ibandronate on the proliferation and the expression of human telomerase reverse transcriptase (hTERT) of non-small cell lung cancer (NSCLC) A549 cell line in vitro. Methods: Methyl thiazolyl tetrazolium (MTT) assay, microscope, flow cytometry (FCM) and semi-quantitative RT-PCR were employed to detect the cell proliferation, cell cycle as well as the morphological change and the expression of hTERT mRNA of A549 cell line. Results: The data showed that ibandronate could effectively inhibit the proliferation of A549 cell line in time- and concentration-dependent. Under the microscope, the floating cells increased gradually as the drug concentration increasing. FCM detection showed that ibandronate could induce the cell cycle stopped in G0/G1 phase and downregulation expression of hTERT. Conclusion: Ibandronate can inhibit the proliferation of A549 cell line in vitro, whose mechanism may be associated with cell cycle arrestted in phase G0/G1 and downregulation expression of hTERT