Intravitreal neurodegenerative and inflammatory mediators in proliferative diabetic retinopathy

ABSTRACT Purpose: To compare the intravitreal concentrations of cellular mediators involved in neurodegeneration, inflammation, and angiogenesis in patients with proliferative diabetic retinopathy and other vitreoretinal diseases. Methods: A multiplex bead immunoassay was used to measure vitreous...

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Main Authors: Felipe Mallmann, Luis Henrique Canani
Format: Article
Language:English
Published: Conselho Brasileiro de Oftalmologia 2019-04-01
Series:Arquivos Brasileiros de Oftalmologia
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492019005006102&lng=en&tlng=en
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spelling doaj-3723bf6947d145f0bf56a10febefa9bc2020-11-25T00:10:48ZengConselho Brasileiro de OftalmologiaArquivos Brasileiros de Oftalmologia1678-29252019-04-01010.5935/0004-2749.20190055S0004-27492019005006102Intravitreal neurodegenerative and inflammatory mediators in proliferative diabetic retinopathyFelipe MallmannLuis Henrique CananiABSTRACT Purpose: To compare the intravitreal concentrations of cellular mediators involved in neurodegeneration, inflammation, and angiogenesis in patients with proliferative diabetic retinopathy and other vitreoretinal diseases. Methods: A multiplex bead immunoassay was used to measure vitreous levels of pigment epithelium-derived factor, serum amyloid P, C-reactive protein, complement C4, alpha-1 antitrypsin, vascular endothelial growth factor, platelet-derived growth factor-AA, platelet-derived growth factor-BB, interleukin-6, interleukin-8, interleukin-10, tumor necrosis factor alpha and beta in patients undergoing 23-gauge vitrectomy for proliferative diabetic retinopathy and other diagnoses (control group). Results: We evaluated 55 patients, of whom 24 had proliferative diabetic retinopathy and 31 had other diagnoses including vitreous hemorrhage, retinal detachment, macular hole, and epiretinal membrane. Patients with proliferative diabetic retinopathy demonstrated increased levels of serum amyloid P (85.49 vs. 31.38 ng/mL); C-reactive protein (59.89 vs. 41.75 ng/mL), vascular endothelial growth factor (2,330.11 vs. 554.25 pg/mL; p<0.001), platelet-derived growth factor A (127.32 vs. 39.11 pg/mL), platelet-derived growth factor B (29.37 vs. 7.12 pg/mL), interleukin-6 (69.37 vs. 33.58 pg/mL), interleukin-8 (175.25 vs. 59.71 pg/mL), and interleukin-10 (3.70 vs. 1.88 pg/mL); all p<0.004 when compared with the control group. Levels of pigment epithelium-derived factor (30.06 vs. 27.48 ng/mL; p=0.295), complement C4 (570.78 vs. 366.24 ng/mL; p=0.069), and alpha-1-antitrypsin (359.27 vs. 522.44 ng/mL; p=0.264) were not significantly different between the groups. Intravitreal levels of tumor necrosis factor-alpha and tumor necrosis factor-beta were undetectable. Serum Amyloid P, C-reactive protein, platelet-derived growth factor A, platelet-derived growth factor B, interleukin-6, and interleukin-8 were correlated positively with vascular endothelial growth factor. Conclusions: Cellular mediators involved in neurodegeneration and inflammation demonstrated increased levels in the vitreous humor of patients with proliferative diabetic retinopathy and may be part of the pathogenesis of diabetic retinopathy.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492019005006102&lng=en&tlng=enRetinopatia diabéticaDegeneração retinianaCitocinasProteínas da fase agudaHumor vítreo
collection DOAJ
language English
format Article
sources DOAJ
author Felipe Mallmann
Luis Henrique Canani
spellingShingle Felipe Mallmann
Luis Henrique Canani
Intravitreal neurodegenerative and inflammatory mediators in proliferative diabetic retinopathy
Arquivos Brasileiros de Oftalmologia
Retinopatia diabética
Degeneração retiniana
Citocinas
Proteínas da fase aguda
Humor vítreo
author_facet Felipe Mallmann
Luis Henrique Canani
author_sort Felipe Mallmann
title Intravitreal neurodegenerative and inflammatory mediators in proliferative diabetic retinopathy
title_short Intravitreal neurodegenerative and inflammatory mediators in proliferative diabetic retinopathy
title_full Intravitreal neurodegenerative and inflammatory mediators in proliferative diabetic retinopathy
title_fullStr Intravitreal neurodegenerative and inflammatory mediators in proliferative diabetic retinopathy
title_full_unstemmed Intravitreal neurodegenerative and inflammatory mediators in proliferative diabetic retinopathy
title_sort intravitreal neurodegenerative and inflammatory mediators in proliferative diabetic retinopathy
publisher Conselho Brasileiro de Oftalmologia
series Arquivos Brasileiros de Oftalmologia
issn 1678-2925
publishDate 2019-04-01
description ABSTRACT Purpose: To compare the intravitreal concentrations of cellular mediators involved in neurodegeneration, inflammation, and angiogenesis in patients with proliferative diabetic retinopathy and other vitreoretinal diseases. Methods: A multiplex bead immunoassay was used to measure vitreous levels of pigment epithelium-derived factor, serum amyloid P, C-reactive protein, complement C4, alpha-1 antitrypsin, vascular endothelial growth factor, platelet-derived growth factor-AA, platelet-derived growth factor-BB, interleukin-6, interleukin-8, interleukin-10, tumor necrosis factor alpha and beta in patients undergoing 23-gauge vitrectomy for proliferative diabetic retinopathy and other diagnoses (control group). Results: We evaluated 55 patients, of whom 24 had proliferative diabetic retinopathy and 31 had other diagnoses including vitreous hemorrhage, retinal detachment, macular hole, and epiretinal membrane. Patients with proliferative diabetic retinopathy demonstrated increased levels of serum amyloid P (85.49 vs. 31.38 ng/mL); C-reactive protein (59.89 vs. 41.75 ng/mL), vascular endothelial growth factor (2,330.11 vs. 554.25 pg/mL; p<0.001), platelet-derived growth factor A (127.32 vs. 39.11 pg/mL), platelet-derived growth factor B (29.37 vs. 7.12 pg/mL), interleukin-6 (69.37 vs. 33.58 pg/mL), interleukin-8 (175.25 vs. 59.71 pg/mL), and interleukin-10 (3.70 vs. 1.88 pg/mL); all p<0.004 when compared with the control group. Levels of pigment epithelium-derived factor (30.06 vs. 27.48 ng/mL; p=0.295), complement C4 (570.78 vs. 366.24 ng/mL; p=0.069), and alpha-1-antitrypsin (359.27 vs. 522.44 ng/mL; p=0.264) were not significantly different between the groups. Intravitreal levels of tumor necrosis factor-alpha and tumor necrosis factor-beta were undetectable. Serum Amyloid P, C-reactive protein, platelet-derived growth factor A, platelet-derived growth factor B, interleukin-6, and interleukin-8 were correlated positively with vascular endothelial growth factor. Conclusions: Cellular mediators involved in neurodegeneration and inflammation demonstrated increased levels in the vitreous humor of patients with proliferative diabetic retinopathy and may be part of the pathogenesis of diabetic retinopathy.
topic Retinopatia diabética
Degeneração retiniana
Citocinas
Proteínas da fase aguda
Humor vítreo
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492019005006102&lng=en&tlng=en
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