MicroRNA-210 interacts with FBXO31 to regulate cancer proliferation cell cycle and migration in human breast cancer

• Main Authors: Liu D, Xia H, Wang F, Chen C, Long J
• Format: Article
• Language: English
• Published: Dove Medical Press 2016-08-01
• Series: OncoTargets and Therapy
• Subjects: Breast cancer; miR-210; FBXO31; cancer proliferation; cancer migration
• Online Access: https://www.dovepress.com/microrna-210-interacts-with-fbxo31-to-regulate-cancer-proliferation-ce-peer-reviewed-article-OTT

Dayue Liu,1,* Haoming Xia,1,* Fang Wang,2 Cui Chen,2 Jianting Long2 1Department of Surgery, Breast Disease Center, 2Department of Medicinal Oncology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People’s Republic of China *These authors contributed equally to this work Background: In this study, we investigated the functional correlation between microRNA-210 (miR-210) and gene of F-box protein 31 (FBXO31) in regulating breast cancer.Methods: Dual-luciferase assay and quantitative real-time polymerase chain reaction were used to investigate the binding of miR-210 with FBXO31 and their expression patterns in breast cancer. miR-210 was inhibited in breast cancer T47D and MCF-7 cells to assess its effect on cancer proliferation, cell cycle progression, and migration. FBXO31 was also downregulated in breast cancer cells to examine its effect on miR-210-mediated breast cancer regulation. The interaction between miR-210 and FBXO31 was further investigated by examining the effect of overexpressing miR-210 on FBXO31-induced suppression of breast cancer proliferation.Results: FBXO31 was the downstream target gene of miR-210 in breast cancer. miR-210 and FBXO31 are inversely expressed in breast cancer cell lines. miR-210 downregulation reduced cancer progression, induced cell cycle arrest, and inhibited cancer migration in T47D and MCF-7 cells. Tumor suppression by miR-210 downregulation was reversed by downregulating FBXO31. In FBXO31-overexpressed breast cancer cells, upregulating miR-210 also reversed the tumor-suppressive effect of FBXO31 on breast cancer proliferation.Conclusion: Our work demonstrated that the expression pattern and tumor regulatory functions of miR-210 and FBXO31 are inversely correlated in breast cancer. Keywords: breast cancer, miR-210, FBXO31, cancer proliferation, cancer migration