Plant endophytic yeasts Pichia fermentans and Meyerozyma caribbica improve growth, biochemical composition, haematological parameters and morphology of internal organs of premature Barbonymus gonionotus

• Main Authors: Fatama Islam, Mohammad Abdus Salam, Md. Ashikur Rahman, Sulav Indra Paul, Tamalika Rani Das, Md. Mahbubur Rahman, Dinesh Chandra Shaha, Dipali Rani Gupta, Md Shah Alam, Tofazzal Islam
• Format: Article
• Language: English
• Published: Elsevier 2021-03-01
• Series: Aquaculture Reports
• Subjects: Endophytic yeasts; Growth; Haematological parameters; Organ histology
• Online Access: http://www.sciencedirect.com/science/article/pii/S2352513420306682

Plant associated yeasts promote growth and protect plants from various pathogens in multifaceted mechanisms. However, no report has so far been published on the effect of plant endophytic yeasts on growth and development of fishes. This study aimed to isolate and identify endophytic yeasts from pineapple (Ananas comosus) and evaluate the effects of these yeasts-enriched diets on growth, nutrients of carcass and mineral contents of muscles, haematological parameters and morphology of intestine, liver and kidney of Barbonymus gonionotus. Two endophytic yeast species viz. Pichia fermentans strain MAS1 and Meyerozyma caribbica strain MAS2 were isolated from A. comosus cultivated at Kapashia upazila of Gazipur district and district of Rangamati in Bangladesh, respectively. The two yeast strains were identified using internal transcribed spacer region (5.8S rRNA gene) sequencing. Both strains MAS1 [(1–3) × 0.94 × 108 CFU kg−1 feed] and MAS2 [(1–3) × 108 CFU kg−1] containing diets were fed to premature B. gonionotus for 60 days and the fish were reared in the plastic tanks with a good aeration system. Fish treated with pineapple yeasts significantly improved growth, nutrients of carcass, mineral contents of the muscles, and haematological parameters of B. gonionotus compared to control. Histological study revealed that the endophytic yeasts significantly enhanced length (μm) of intestinal villi compared to control fishes. Furthermore, the yeast treated formulated diet considerably reduced the fat content in liver and also improved the function of the kidney of B. gonionotus compared to the control group. Among the treatments, application of strain MAS1 at 0.94 × 108 CFU kg−1 feed showed the highest beneficial effects to B. gonionotus indicating its potentials for practical application in promoting sustainable and safe aquaculture.