Detection of genetic variation and base modifications at base-pair resolution on both DNA and RNA
Wang et al. show how genetic sequence and base modifications can be detected simultaneously on single molecules of both DNA and RNA using magnetic tweezers. They also demonstrate an amplification-free CRISPR/Cas9-based strategy for isolating target regions from native DNA and apply this approach to...
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| Language: | English |
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Nature Publishing Group
2021-01-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-021-01648-7 |
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doaj-36f2d6c25b90460cab0986d3713e402b2021-01-31T16:16:34ZengNature Publishing GroupCommunications Biology2399-36422021-01-014111410.1038/s42003-021-01648-7Detection of genetic variation and base modifications at base-pair resolution on both DNA and RNAZhen Wang0Jérôme Maluenda1Laurène Giraut2Thibault Vieille3Andréas Lefevre4David Salthouse5Gaël Radou6Rémi Moulinas7Sandra Astete8Pol D’Avezac9Geoff Smith10Charles André11Jean-François Allemand12David Bensimon13Vincent Croquette14Jimmy Ouellet15Gordon Hamilton16Depixus SAS, 3/5 Impasse ReilleDepixus SAS, 3/5 Impasse ReilleDepixus SAS, 3/5 Impasse ReilleDepixus SAS, 3/5 Impasse ReilleDepixus SAS, 3/5 Impasse ReilleDepixus SAS, 3/5 Impasse ReilleDepixus SAS, 3/5 Impasse ReilleDepixus SAS, 3/5 Impasse ReilleDepixus SAS, 3/5 Impasse ReilleDepixus SAS, 3/5 Impasse ReilleDepixus SAS, 3/5 Impasse ReilleDepixus SAS, 3/5 Impasse ReilleLaboratoire de physique de L’École normale supérieure de Paris, CNRS, ENS, Université PSL, Sorbonne Université, Université de ParisLaboratoire de physique de L’École normale supérieure de Paris, CNRS, ENS, Université PSL, Sorbonne Université, Université de ParisLaboratoire de physique de L’École normale supérieure de Paris, CNRS, ENS, Université PSL, Sorbonne Université, Université de ParisDepixus SAS, 3/5 Impasse ReilleDepixus SAS, 3/5 Impasse ReilleWang et al. show how genetic sequence and base modifications can be detected simultaneously on single molecules of both DNA and RNA using magnetic tweezers. They also demonstrate an amplification-free CRISPR/Cas9-based strategy for isolating target regions from native DNA and apply this approach to the isolation of targets from E. coli and human genomic DNA.https://doi.org/10.1038/s42003-021-01648-7 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Zhen Wang Jérôme Maluenda Laurène Giraut Thibault Vieille Andréas Lefevre David Salthouse Gaël Radou Rémi Moulinas Sandra Astete Pol D’Avezac Geoff Smith Charles André Jean-François Allemand David Bensimon Vincent Croquette Jimmy Ouellet Gordon Hamilton |
| spellingShingle |
Zhen Wang Jérôme Maluenda Laurène Giraut Thibault Vieille Andréas Lefevre David Salthouse Gaël Radou Rémi Moulinas Sandra Astete Pol D’Avezac Geoff Smith Charles André Jean-François Allemand David Bensimon Vincent Croquette Jimmy Ouellet Gordon Hamilton Detection of genetic variation and base modifications at base-pair resolution on both DNA and RNA Communications Biology |
| author_facet |
Zhen Wang Jérôme Maluenda Laurène Giraut Thibault Vieille Andréas Lefevre David Salthouse Gaël Radou Rémi Moulinas Sandra Astete Pol D’Avezac Geoff Smith Charles André Jean-François Allemand David Bensimon Vincent Croquette Jimmy Ouellet Gordon Hamilton |
| author_sort |
Zhen Wang |
| title |
Detection of genetic variation and base modifications at base-pair resolution on both DNA and RNA |
| title_short |
Detection of genetic variation and base modifications at base-pair resolution on both DNA and RNA |
| title_full |
Detection of genetic variation and base modifications at base-pair resolution on both DNA and RNA |
| title_fullStr |
Detection of genetic variation and base modifications at base-pair resolution on both DNA and RNA |
| title_full_unstemmed |
Detection of genetic variation and base modifications at base-pair resolution on both DNA and RNA |
| title_sort |
detection of genetic variation and base modifications at base-pair resolution on both dna and rna |
| publisher |
Nature Publishing Group |
| series |
Communications Biology |
| issn |
2399-3642 |
| publishDate |
2021-01-01 |
| description |
Wang et al. show how genetic sequence and base modifications can be detected simultaneously on single molecules of both DNA and RNA using magnetic tweezers. They also demonstrate an amplification-free CRISPR/Cas9-based strategy for isolating target regions from native DNA and apply this approach to the isolation of targets from E. coli and human genomic DNA. |
| url |
https://doi.org/10.1038/s42003-021-01648-7 |
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