Application of fast light-readapted plants for measurement of chlorophyll fluorescence and P700 light absorption with the RLC method
The rapid light curve method is a time-saving approach to the measurement of photosynthetic processes over a wide spectrum of light intensities. The adaptation to darkness enables the calculation of a complete set of coefficients for chlorophyll (Chl) fluorescence and P700 light absorption; however,...
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| Format: | Article |
| Language: | English |
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Academy of Sciences of the Czech Republic, Institute of Experimental Botany
2021-06-01
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| Series: | Photosynthetica |
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| Online Access: | https://ps.ueb.cas.cz/artkey/phs-202102-0003_application-of-fast-light-readapted-plants-for-measurement-of-chlorophyll-fluorescence-and-p700-light-absorptio.php |
| Summary: | The rapid light curve method is a time-saving approach to the measurement of photosynthetic processes over a wide spectrum of light intensities. The adaptation to darkness enables the calculation of a complete set of coefficients for chlorophyll (Chl) fluorescence and P700 light absorption; however, dark-adapted plants demonstrate drastically different patterns of Chl fluorescence than that of light-adapted plants. The present work compared light-adapted barley plants without dark adaptation (L), and barley plants adapted to darkness and readapted to light for 7.5 min (D/L). The rapid light curves were generally similar in both the L and D/L variants. Some differences between the L and D/L variants were found in plants grown at any illumination. A number of distinctions between the L and D/L variants were characteristic of plants grown under particular illumination levels. The present analysis demonstrated that the scheme including dark adaptation and short readaptation to light (D/L) enabled to obtain generally correct data. |
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| ISSN: | 0300-3604 0300-3604 |