Bone Status in a Mouse Model of Experimental Autoimmune-Orchitis

• Main Authors: Fabian Hemm, Monika Fijak, Jan Belikan, Marian Kampschulte, Thaqif El Khassawna, Adrian Pilatz, Christian Heiss, Katrin Susanne Lips
• Format: Article
• Language: English
• Published: MDPI AG 2021-07-01
• Series: International Journal of Molecular Sciences
• Subjects: experimental autoimmune-orchitis; osteoporosis; mouse model; micro-CT; biomechanical properties; testosterone
• Online Access: https://www.mdpi.com/1422-0067/22/15/7858
• ISSN: 1661-6596; 1422-0067

Investigations in male patients with fertility disorders revealed a greater risk of osteoporosis. The rodent model of experimental autoimmune-orchitis (EAO) was established to analyze the underlying mechanisms of male infertility and causes of reduced testosterone concentration. Hence, we investigated the impact of testicular dysfunction in EAO on bone status. Male mice were immunized with testicular homogenate in adjuvant to induce EAO (<i>n</i> = 5). Age-matched mice were treated with adjuvant alone (adjuvant, <i>n</i> = 6) or remained untreated (control, <i>n</i> = 7). Fifty days after the first immunization specimens were harvested. Real-time reverse transcription-PCR indicated decreased bone metabolism by alkaline phosphatase and Cathepsin K as well as remodeling of cell-contacts by Connexin-43. Micro computed tomography demonstrated a loss of bone mass and mineralization. These findings were supported by histomorphometric results. Additionally, biomechanical properties of femora in a three-point bending test were significantly altered. In summary, the present study illustrates the induction of osteoporosis in the investigated mouse model. However, results suggest that the major effects on bone status were mainly caused by the complete Freund’s adjuvant rather than the autoimmune-orchitis itself. Therefore, the benefit of the EAO model to transfer laboratory findings regarding bone metabolism in context with orchitis into a clinical application is limited.