PCR data and comparative performance of Bacteroidales microbial source tracking genetic markers

PCR data and comparative performance of Bacteroidales microbial source tracking genetic markers

We reported modified endpoint PCR results analyzed by universal and human-, swine-, and cattle-specific Bacteroidales gene markers with human sewage and animal fecal samples (i.e., swine, cattle, chicken, goat, sheep, buffalo, and duck) from Tha Chin and Chao Phraya watersheds. Annealing locations o...

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Main Authors: Pornjira Somnark, Natcha Chyerochana, Akechai Kongprajug, Skorn Mongkolsuk, Kwanrawee Sirikanchana
Format: Article
Language:English
Published: Elsevier 2018-08-01
Series:Data in Brief
Online Access:http://www.sciencedirect.com/science/article/pii/S2352340918304827
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spelling doaj-36b54f9f84e6440b9ddd800be30d299c2020-11-25T02:53:08ZengElsevierData in Brief2352-34092018-08-0119156169PCR data and comparative performance of Bacteroidales microbial source tracking genetic markersPornjira Somnark0Natcha Chyerochana1Akechai Kongprajug2Skorn Mongkolsuk3Kwanrawee Sirikanchana4Applied Biological Sciences, Chulabhorn Graduate Institute, Chulabhorn Royal Academy, Lak Si, Bangkok 10210 ThailandResearch Laboratory of Biotechnology, Chulabhorn Research Institute, Lak Si, Bangkok 10210 ThailandResearch Laboratory of Biotechnology, Chulabhorn Research Institute, Lak Si, Bangkok 10210 ThailandResearch Laboratory of Biotechnology, Chulabhorn Research Institute, Lak Si, Bangkok 10210 Thailand; Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400 Thailand; Center of Excellence on Environmental Health and Toxicology (EHT), Ministry of Education, Bangkok 10210 ThailandResearch Laboratory of Biotechnology, Chulabhorn Research Institute, Lak Si, Bangkok 10210 Thailand; Center of Excellence on Environmental Health and Toxicology (EHT), Ministry of Education, Bangkok 10210 Thailand; Corresponding author at: Research Laboratory of Biotechnology, Chulabhorn Research Institute, Lak Si, Bangkok, Thailand.We reported modified endpoint PCR results analyzed by universal and human-, swine-, and cattle-specific Bacteroidales gene markers with human sewage and animal fecal samples (i.e., swine, cattle, chicken, goat, sheep, buffalo, and duck) from Tha Chin and Chao Phraya watersheds. Annealing locations of PCR primers were illustrated by maps of 16s rRNA Bacteroidales genes. We also summarized previously published work on the performance of the PCR assays. For further discussion of the data presented here, please refer to Somnark et al., Performance evaluation of Bacteroidales genetic markers for human and animal microbial source tracking in tropical agricultural watersheds, Environ. Pollut. 236 (2018) 100–110. Keywords: Endpoint PCR, Fecal pollution, Microbial source tracking, Bacteroidales, Sensitivity, Specificity, Water qualityhttp://www.sciencedirect.com/science/article/pii/S2352340918304827
collection DOAJ
language English
format Article
sources DOAJ
author Pornjira Somnark
Natcha Chyerochana
Akechai Kongprajug
Skorn Mongkolsuk
Kwanrawee Sirikanchana
spellingShingle Pornjira Somnark
Natcha Chyerochana
Akechai Kongprajug
Skorn Mongkolsuk
Kwanrawee Sirikanchana
PCR data and comparative performance of Bacteroidales microbial source tracking genetic markers
Data in Brief
author_facet Pornjira Somnark
Natcha Chyerochana
Akechai Kongprajug
Skorn Mongkolsuk
Kwanrawee Sirikanchana
author_sort Pornjira Somnark
title PCR data and comparative performance of Bacteroidales microbial source tracking genetic markers
title_short PCR data and comparative performance of Bacteroidales microbial source tracking genetic markers
title_full PCR data and comparative performance of Bacteroidales microbial source tracking genetic markers
title_fullStr PCR data and comparative performance of Bacteroidales microbial source tracking genetic markers
title_full_unstemmed PCR data and comparative performance of Bacteroidales microbial source tracking genetic markers
title_sort pcr data and comparative performance of bacteroidales microbial source tracking genetic markers
publisher Elsevier
series Data in Brief
issn 2352-3409
publishDate 2018-08-01
description We reported modified endpoint PCR results analyzed by universal and human-, swine-, and cattle-specific Bacteroidales gene markers with human sewage and animal fecal samples (i.e., swine, cattle, chicken, goat, sheep, buffalo, and duck) from Tha Chin and Chao Phraya watersheds. Annealing locations of PCR primers were illustrated by maps of 16s rRNA Bacteroidales genes. We also summarized previously published work on the performance of the PCR assays. For further discussion of the data presented here, please refer to Somnark et al., Performance evaluation of Bacteroidales genetic markers for human and animal microbial source tracking in tropical agricultural watersheds, Environ. Pollut. 236 (2018) 100–110. Keywords: Endpoint PCR, Fecal pollution, Microbial source tracking, Bacteroidales, Sensitivity, Specificity, Water quality
url http://www.sciencedirect.com/science/article/pii/S2352340918304827
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