Inclusion of Antibodies to Cell Culture Media Preserves the Integrity of Genes Encoding RL13 and the Pentameric Complex Components During Fibroblast Passage of Human Cytomegalovirus

Inclusion of Antibodies to Cell Culture Media Preserves the Integrity of Genes Encoding RL13 and the Pentameric Complex Components During Fibroblast Passage of Human Cytomegalovirus

Propagation of human cytomegalovirus (CMV) in cultured cells results in genetic adaptations that confer improved growth in vitro and significant attenuation in vivo. Mutations in RL13 arise quickly, while mutations in the UL128-131A locus emerge later during fibroblast passage and disrupt formation...

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Bibliographic Details
Main Authors: Amine Ourahmane, Xiaohong Cui, Li He, Meaghan Catron, Dirk P. Dittmer, Ahmed Al Qaffasaa, Mark R. Schleiss, Laura Hertel, Michael A. McVoy
Format: Article
Language:English
Published: MDPI AG 2019-03-01
Series:Viruses
Subjects:
cytomegalovirus
cell culture
antibodies
adaptation
Online Access:http://www.mdpi.com/1999-4915/11/3/221

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