Diethyl Phthalate Causes Oxidative Stress: An in Vitro Study

• Main Authors: Heena Prajapati, Ramtej Jayram Verma
• Format: Article
• Language: English
• Published: Arak University of Medical Sciences 2014-03-01
• Series: Iranian Journal of Toxicology
• Subjects: Diethyl Phthalate; Lipid Peroxidation; Oxidative Stress
• Online Access: http://ijt.arakmu.ac.ir/browse.php?a_id=308&sid=1&slc_lang=en

Background: Phthalates are a group of multifunctional chemicals. Diethyl phthalate (DEP) is one of the most frequently used phthalates in solvents and fixatives for numerous industrial products. Method: The present experiment was designed to assess oxidative stress, if any, caused by diethyl phthalate. For this the homogenates of liver and kidney were treated with different concentrations ( 10-40 µg/mL) of DEP. 10% liver and kidney homogenates were prepared in phosphate buffered saline and used for estimation of lipid peroxidation.In final step lipid peroxidation and total protein content were analyzed. Results: The result revealed significant and dose - dependent increase in lipid peroxidation, whereas protein content reduced significantly. Maximum increase in LPO and decrease in protein content was observed at 40 µg/mL of DEP concentration. Conclusion: From this study, it can be concluded that different concentrations of DEP leads to dose- dependent significant increase in lipid peroxidation and decrease protein content.So at the different concentration of DEP cause oxidative stress.