Diethyl Phthalate Causes Oxidative Stress: An in Vitro Study
Background: Phthalates are a group of multifunctional chemicals. Diethyl phthalate (DEP) is one of the most frequently used phthalates in solvents and fixatives for numerous industrial products. Method: The present experiment was designed to assess oxidative stress, if any, caused by diethyl phtha...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Arak University of Medical Sciences
2014-03-01
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| Series: | Iranian Journal of Toxicology |
| Subjects: | |
| Online Access: | http://ijt.arakmu.ac.ir/browse.php?a_id=308&sid=1&slc_lang=en |
| Summary: | Background: Phthalates are a group of multifunctional chemicals. Diethyl phthalate (DEP) is one of the most frequently used phthalates in solvents and fixatives for numerous industrial products.
Method: The present experiment was designed to assess oxidative stress, if any, caused by diethyl phthalate. For this the homogenates of liver and kidney were treated with different concentrations ( 10-40 µg/mL) of DEP. 10% liver and kidney homogenates were prepared in phosphate buffered saline and used for estimation of lipid peroxidation.In final step lipid peroxidation and total protein content were analyzed.
Results: The result revealed significant and dose - dependent increase in lipid peroxidation, whereas protein content reduced significantly. Maximum increase in LPO and decrease in protein content was observed at 40 µg/mL of DEP concentration.
Conclusion: From this study, it can be concluded that different concentrations of DEP leads to dose- dependent significant increase in lipid peroxidation and decrease protein content.So at the different concentration of DEP cause oxidative stress.
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| ISSN: | 2008-2967 2251-9459 |