Simultaneous High Performance Liquid Chromatography Assay of Pentoxifylline, Mupirocin, Itraconazole, and Fluticasone Propionate in Humco™ Lavare Wound Base

• Main Author: Troy Purvis
• Format: Article
• Language: English
• Published: MDPI AG 2015-11-01
• Series: Chromatography
• Subjects: Pentoxifylline; Mupirocin; Itraconazole; Fluticasone Propionate; high performance liquid chromatography (HPLC); Humco™ Lavare wound base
• Online Access: http://www.mdpi.com/2227-9075/2/4/642

This article details the elements used in the method verification for the simultaneous high performance liquid chromatography (HPLC) assay of Pentoxifylline, Mupirocin, Itraconazole, and Fluticasone Propionate in Humco™ Lavare Wound base. The method was proven to be linear over 50%–150% of the nominal concentration of the standards. The method was proven to be accurate over 50%–150%, with 98%–102% recovery of the actives from spiked placeboes over that range. The method was shown to be specific to the analytes listed and precise, yielding acceptable results for system reproducibility and method repeatability. The method, as written, is considered to have been verified.