Method validation and stability study of quercetin in topical emulsions

• Main Authors: Rúbia Casagrande, Marcela M. Baracat, Sandra R. Georgetti, Waldiceu A. Verri Jr, Fabiana T. M. C. Vicentini, Janice A. Rafael, José R. Jabor, Maria J. V. Fonseca
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Química 2009-01-01
• Series: Química Nova
• Subjects: quercetin; HPLC; validation
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000700041

This study validated a high performance liquid chromatography (HPLC) method for the quantitative evaluation of quercetin in topical emulsions. The method was linear within 0.05 - 200 μg/mL range with a correlation coefficient of 0.9997, and without interference in the quercetin peak. The detection and quantitation limits were 18 and 29 ng/mL, respectively. The intra- and inter-assay precisions presented R.S.D. values lower than 2%. An average of 93% and 94% of quercetin was recovered for non-ionic and anionic emulsions, respectively. The raw material and anionic emulsion, but not non-ionic emulsion, were stable in all storage conditions for one year. The method reported is a fast and reliable HPLC technique useful for quercetin determination in topical emulsions.