Advantages of Sheep Infrapatellar Fat Pad Adipose Tissue Derived Stem Cells in Tissue Engineering
Purpose: The goal of this study has been to evaluate adipose tissue derived stem cells (ADSCs) from infrapatellar fat pad and characterize their cell surface markers using anti-human antibodies, as adipose tissue derived stem cells (ADSCs) have great potential for cellular therapies to restore injur...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Tabriz University of Medical Sciences
2016-03-01
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| Series: | Advanced Pharmaceutical Bulletin |
| Subjects: | |
| Online Access: | http://journals.tbzmed.ac.ir/APB/Manuscript/APB-6-105.pdf |
| Summary: | Purpose: The goal of this study has
been to evaluate adipose tissue derived stem cells (ADSCs) from
infrapatellar fat pad and characterize their cell surface markers using
anti-human antibodies, as adipose tissue derived stem cells (ADSCs)
have great potential for cellular therapies to restore injured tissues.
Methods: Adipose
tissue was obtained from infrapatellar fat pad of sheep. Surface markers
evaluated by flow cytometry. In order to evaluate
cell adhesion, the Polycaprolactone (PCL)
was sterilized under Ultraviolet (UV) light and about 1×105 cells
were seeded on PCL. Then, ASCs- PCL construct were evaluated by Scanning Electron
Microscopy (Mira3 Te Scan, Czech Republic).
Results: We showed
that adipose tissue derived stem cells (ADSCs) maintain their
fibroblastic-like morphology during different subcultures and cell adhesion.
They were positive for CD44 and CD90 markers and negative for CD31 and Cd45
markers by human antibodies.
Conclusion: Our
results suggest that ASCs surface markers can be characterized by anti-human
antibodies in sheep. As stem cells, they can be used in tissue engineering. |
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| ISSN: | 2228-5881 2251-7308 |