Data on single-step purification method for dye-labeled DNA sequencing

Data on single-step purification method for dye-labeled DNA sequencing

Dye-labelled DNA sequencing is one of the most common and robust technique required for molecular biology since 1977 (Sanger, 1977) [1]. I have recently provided the single-step purification method for dye-labeled sequencing products, which is based on the removal of the washing step in EDTA/ethanol...

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Main Author: Kohei Fujikura
Format: Article
Language:English
Published: Elsevier 2016-06-01
Series:Data in Brief
Online Access:http://www.sciencedirect.com/science/article/pii/S2352340916300725
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spelling doaj-35fef1c80f8d432a8ed654f8b7c6d87a2020-11-25T01:45:05ZengElsevierData in Brief2352-34092016-06-017873876Data on single-step purification method for dye-labeled DNA sequencingKohei Fujikura0Kobe University School of Medicine, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe 650-0017, JapanDye-labelled DNA sequencing is one of the most common and robust technique required for molecular biology since 1977 (Sanger, 1977) [1]. I have recently provided the single-step purification method for dye-labeled sequencing products, which is based on the removal of the washing step in EDTA/ethanol precipitation (Fujikura, 2015) [2]. Here I assess and report the accumulated data of the modified method on the larger scale in practice. Keywords: DNA sequencing, Purification, Ethanol precipitation, EDTA, Quality valuehttp://www.sciencedirect.com/science/article/pii/S2352340916300725
collection DOAJ
language English
format Article
sources DOAJ
author Kohei Fujikura
spellingShingle Kohei Fujikura
Data on single-step purification method for dye-labeled DNA sequencing
Data in Brief
author_facet Kohei Fujikura
author_sort Kohei Fujikura
title Data on single-step purification method for dye-labeled DNA sequencing
title_short Data on single-step purification method for dye-labeled DNA sequencing
title_full Data on single-step purification method for dye-labeled DNA sequencing
title_fullStr Data on single-step purification method for dye-labeled DNA sequencing
title_full_unstemmed Data on single-step purification method for dye-labeled DNA sequencing
title_sort data on single-step purification method for dye-labeled dna sequencing
publisher Elsevier
series Data in Brief
issn 2352-3409
publishDate 2016-06-01
description Dye-labelled DNA sequencing is one of the most common and robust technique required for molecular biology since 1977 (Sanger, 1977) [1]. I have recently provided the single-step purification method for dye-labeled sequencing products, which is based on the removal of the washing step in EDTA/ethanol precipitation (Fujikura, 2015) [2]. Here I assess and report the accumulated data of the modified method on the larger scale in practice. Keywords: DNA sequencing, Purification, Ethanol precipitation, EDTA, Quality value
url http://www.sciencedirect.com/science/article/pii/S2352340916300725
work_keys_str_mv AT koheifujikura dataonsinglesteppurificationmethodfordyelabeleddnasequencing
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