Antioxidant and Anti-Inflammatory Effect and Probiotic Properties of Lactic Acid Bacteria Isolated from Canine and Feline Feces

• Main Authors: Ki-Tae Kim, Jin-Woo Kim, Sun-Il Kim, Seonyoung Kim, Trung Hau Nguyen, Chang-Ho Kang
• Format: Article
• Language: English
• Published: MDPI AG 2021-09-01
• Series: Microorganisms
• Subjects: lactic acid bacteria; antioxidant; anti-inflammatory; canine; feline
• Online Access: https://www.mdpi.com/2076-2607/9/9/1971

Oxidative stress is a phenomenon caused by an imbalance between the production of reactive oxygen species and antioxidant defenses. It plays an important role in numerous disease states, including chronic kidney disease, neurological disorders, cardiovascular diseases, diabetes, and cancer. Lactic acid bacteria (LAB) are known to have prominent antioxidant properties. Therefore, this study aimed to measure the antioxidant activity and anti-inflammatory potential of LAB isolated from animals for the efficient use of probiotics with host specificity. Antioxidant activity measurements of sixteen strains revealed that ABTS radical scavenging activities ranged from 26.3 to 57.4%, and DPPH free radical scavenging activities ranged from 4.7 to 13.5%. Based on the antioxidant activity assessment, five strains (<i>Enterococcus faecium</i> MG9003(YH9003)<i>, Enterococcus faecium</i> MG9007(YH9007), <i>Lactobacillus reuteri</i> MG9012(YH9012), <i>Lactobacillus fermentum</i> MG9014(YH9014), and <i>Pediococcus pentosaceus</i> MG9015(YH9015)) were selected with the consideration of fermentation productivity (>1 × 10⁹ CFU/g). The selected strains exhibited nitric oxide inhibition and inhibited inducible nitric oxide synthase and cyclooxygenase expression. Furthermore, probiotic properties, including intestinal adhesion and stability, were identified. Our results show that the selected animal-derived strains can be effective probiotic candidates for potential effects on animal hosts.