A Novel Route for Agarooligosaccharide Production with the Neoagarooligosaccharide-Producing β-Agarase as Catalyst

Enzymes are catalysts with high specificity. Different compounds could be produced by different enzymes. In case of agaro-oligosaccharides, agarooligosaccharide (AOS) can be produced by α-agarase through cleaving the α-1,3-glycosidic linkages of agarose, while neoagarooligosacchari...

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Main Authors:	Chengcheng Jiang, Zhen Liu, Jianan Sun, Changhu Xue, Xiangzhao Mao
Format:	Article
Language:	English
Published:	MDPI AG 2020-02-01
Series:	Catalysts
Subjects:	agarose agarase agarotriose agaropentaose expression
Online Access:	https://www.mdpi.com/2073-4344/10/2/214

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record_format	Article
spelling	doaj-35dbbddfcbfe435489b97531794569ba2020-11-25T01:40:01ZengMDPI AGCatalysts2073-43442020-02-0110221410.3390/catal10020214catal10020214A Novel Route for Agarooligosaccharide Production with the Neoagarooligosaccharide-Producing β-Agarase as CatalystChengcheng Jiang0Zhen Liu1Jianan Sun2Changhu Xue3Xiangzhao Mao4College of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaEnzymes are catalysts with high specificity. Different compounds could be produced by different enzymes. In case of agaro-oligosaccharides, agarooligosaccharide (AOS) can be produced by α-agarase through cleaving the α-1,3-glycosidic linkages of agarose, while neoagarooligosaccharide (NAOS) can be produced by β-agarase through cleaving the β-1,4-glycosidic linkages of agarose. However, in this study, we showed that β-agarase could also be used to produce AOSs with high purity and yield. The feasibility of our route was confirmed by agarotriose (A3) and agaropentaose (A5) formation from agaroheptaose (A7) and agarononoses (A9) catalyzed by β-agarase. Agarose was firstly liquesced by citric acid into a mixture of AOSs. The AOSs mixture was further catalyzed by β-agarase. When using the neoagarotetraose-forming β-agarase AgWH50B, agarotriose could be produced with the yield of 48%. When using neoagarotetraose, neoagarohexaose-forming β-agarase DagA, both agarotriose and agaropentaose could be produced with the yield of 14% and 13%, respectively. Our method can be used to produce other value-added agaro-oligosaccharides from agarose by different agarolytic enzymes.https://www.mdpi.com/2073-4344/10/2/214agaroseagaraseagarotrioseagaropentaoseexpression
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Chengcheng Jiang Zhen Liu Jianan Sun Changhu Xue Xiangzhao Mao
spellingShingle	Chengcheng Jiang Zhen Liu Jianan Sun Changhu Xue Xiangzhao Mao A Novel Route for Agarooligosaccharide Production with the Neoagarooligosaccharide-Producing β-Agarase as Catalyst Catalysts agarose agarase agarotriose agaropentaose expression
author_facet	Chengcheng Jiang Zhen Liu Jianan Sun Changhu Xue Xiangzhao Mao
author_sort	Chengcheng Jiang
title	A Novel Route for Agarooligosaccharide Production with the Neoagarooligosaccharide-Producing β-Agarase as Catalyst
title_short	A Novel Route for Agarooligosaccharide Production with the Neoagarooligosaccharide-Producing β-Agarase as Catalyst
title_full	A Novel Route for Agarooligosaccharide Production with the Neoagarooligosaccharide-Producing β-Agarase as Catalyst
title_fullStr	A Novel Route for Agarooligosaccharide Production with the Neoagarooligosaccharide-Producing β-Agarase as Catalyst
title_full_unstemmed	A Novel Route for Agarooligosaccharide Production with the Neoagarooligosaccharide-Producing β-Agarase as Catalyst
title_sort	novel route for agarooligosaccharide production with the neoagarooligosaccharide-producing β-agarase as catalyst
publisher	MDPI AG
series	Catalysts
issn	2073-4344
publishDate	2020-02-01
description	Enzymes are catalysts with high specificity. Different compounds could be produced by different enzymes. In case of agaro-oligosaccharides, agarooligosaccharide (AOS) can be produced by α-agarase through cleaving the α-1,3-glycosidic linkages of agarose, while neoagarooligosaccharide (NAOS) can be produced by β-agarase through cleaving the β-1,4-glycosidic linkages of agarose. However, in this study, we showed that β-agarase could also be used to produce AOSs with high purity and yield. The feasibility of our route was confirmed by agarotriose (A3) and agaropentaose (A5) formation from agaroheptaose (A7) and agarononoses (A9) catalyzed by β-agarase. Agarose was firstly liquesced by citric acid into a mixture of AOSs. The AOSs mixture was further catalyzed by β-agarase. When using the neoagarotetraose-forming β-agarase AgWH50B, agarotriose could be produced with the yield of 48%. When using neoagarotetraose, neoagarohexaose-forming β-agarase DagA, both agarotriose and agaropentaose could be produced with the yield of 14% and 13%, respectively. Our method can be used to produce other value-added agaro-oligosaccharides from agarose by different agarolytic enzymes.
topic	agarose agarase agarotriose agaropentaose expression
url	https://www.mdpi.com/2073-4344/10/2/214
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A Novel Route for Agarooligosaccharide Production with the Neoagarooligosaccharide-Producing β-Agarase as Catalyst

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