NBD-Based Environment-Sensitive Fluorescent Probes for the Human Ether-a-Go-Go–Related Gene Potassium Channel

Three environment-sensitive probes were developed for the hERG channel based on the nitrobenzoxadiazole fluorophore herein. After careful evaluation, probes M1 and M3 were found to have a high affinity for imaging the hERG channel in the cell-based experiment. Compared with other fluorescent labelin...

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Main Authors:	Qi Li, Lijuan Chai, Gaopan Dong, Xiaomeng Zhang, Lupei Du
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2021-05-01
Series:	Frontiers in Molecular Biosciences
Subjects:	hERG potassium channel fluorescent labeling technology small-molecule fluorescent probe fluorescent imaging cell imaging
Online Access:	https://www.frontiersin.org/articles/10.3389/fmolb.2021.666605/full

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record_format	Article
spelling	doaj-35c75800d9a543b8a79110ff21b1e6822021-05-14T06:42:06ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2021-05-01810.3389/fmolb.2021.666605666605NBD-Based Environment-Sensitive Fluorescent Probes for the Human Ether-a-Go-Go–Related Gene Potassium ChannelQi Li0Lijuan Chai1Gaopan Dong2Xiaomeng Zhang3Lupei Du4Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (MOE), School of Pharmacy, Shandong University, Jinan, ChinaDepartment of Internal Medicine, Hospital of Shandong University, Jinan, ChinaDepartment of Medicinal Chemistry, Key Laboratory of Chemical Biology (MOE), School of Pharmacy, Shandong University, Jinan, ChinaDepartment of Medicinal Chemistry, Key Laboratory of Chemical Biology (MOE), School of Pharmacy, Shandong University, Jinan, ChinaDepartment of Medicinal Chemistry, Key Laboratory of Chemical Biology (MOE), School of Pharmacy, Shandong University, Jinan, ChinaThree environment-sensitive probes were developed for the hERG channel based on the nitrobenzoxadiazole fluorophore herein. After careful evaluation, probes M1 and M3 were found to have a high affinity for imaging the hERG channel in the cell-based experiment. Compared with other fluorescent labeling technologies (such as fluorescent proteins), these probes afford a convenient and economical method to determine hERG channel in vitro and in cellulo. Therefore, these probes are expected to be applicable for usage in physiological and pathological studies of hERG channels and have the potential to establish a screening system for hERG channels.https://www.frontiersin.org/articles/10.3389/fmolb.2021.666605/fullhERG potassium channelfluorescent labeling technologysmall-molecule fluorescent probefluorescent imagingcell imaging
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Qi Li Lijuan Chai Gaopan Dong Xiaomeng Zhang Lupei Du
spellingShingle	Qi Li Lijuan Chai Gaopan Dong Xiaomeng Zhang Lupei Du NBD-Based Environment-Sensitive Fluorescent Probes for the Human Ether-a-Go-Go–Related Gene Potassium Channel Frontiers in Molecular Biosciences hERG potassium channel fluorescent labeling technology small-molecule fluorescent probe fluorescent imaging cell imaging
author_facet	Qi Li Lijuan Chai Gaopan Dong Xiaomeng Zhang Lupei Du
author_sort	Qi Li
title	NBD-Based Environment-Sensitive Fluorescent Probes for the Human Ether-a-Go-Go–Related Gene Potassium Channel
title_short	NBD-Based Environment-Sensitive Fluorescent Probes for the Human Ether-a-Go-Go–Related Gene Potassium Channel
title_full	NBD-Based Environment-Sensitive Fluorescent Probes for the Human Ether-a-Go-Go–Related Gene Potassium Channel
title_fullStr	NBD-Based Environment-Sensitive Fluorescent Probes for the Human Ether-a-Go-Go–Related Gene Potassium Channel
title_full_unstemmed	NBD-Based Environment-Sensitive Fluorescent Probes for the Human Ether-a-Go-Go–Related Gene Potassium Channel
title_sort	nbd-based environment-sensitive fluorescent probes for the human ether-a-go-go–related gene potassium channel
publisher	Frontiers Media S.A.
series	Frontiers in Molecular Biosciences
issn	2296-889X
publishDate	2021-05-01
description	Three environment-sensitive probes were developed for the hERG channel based on the nitrobenzoxadiazole fluorophore herein. After careful evaluation, probes M1 and M3 were found to have a high affinity for imaging the hERG channel in the cell-based experiment. Compared with other fluorescent labeling technologies (such as fluorescent proteins), these probes afford a convenient and economical method to determine hERG channel in vitro and in cellulo. Therefore, these probes are expected to be applicable for usage in physiological and pathological studies of hERG channels and have the potential to establish a screening system for hERG channels.
topic	hERG potassium channel fluorescent labeling technology small-molecule fluorescent probe fluorescent imaging cell imaging
url	https://www.frontiersin.org/articles/10.3389/fmolb.2021.666605/full
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NBD-Based Environment-Sensitive Fluorescent Probes for the Human Ether-a-Go-Go–Related Gene Potassium Channel

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