Effect of non-autologous adipose-derived stem cells transplantation and nerve growth factor on the repair of crushed sciatic nerve in rats

• Main Authors: Azadeh Tajik, Mahnaz Azarnia, Gholamreza Kaka, Seyed Homayoon Sadraie, Amid Khatiby Aghda
• Format: Article
• Language: fas
• Published: Birjand University of Medical Sciences and Health Services 2014-02-01
• Series: Journal of Birjand University of Medical Sciences
• Subjects: Adipose-derived stem cell (ADSc); Nerve growth factor (NGF); Nerve Repair
• Online Access: http://journal.bums.ac.ir/browse.php?a_code=A-10-1134-1&slc_lang=en&sid=1
• ISSN: 1607-2197; 2423-6152

Background and Aim: Application of stem cells derived from adipose tissue is very easy. The present study evaluated the effect of transplantation of non-autolugus of adipose derived stem cell (ADSc) and nerve growth factor (NGF) in repairing of crushed sciatic nerve in rats by examining their behavior and electrophysiology. Materials and Methods: Twenty-eight adult male rats were used in this study. The animals were randomly divided into four equal groups: (healthy rats), as control(sciatic nerve crushed rats), (sciatic nerve crushed rats treated with NGF), and (sciatic nerve crushed rats treated by means of transplantation of non-autolugue of ADSc).Then,the improvement of sensory function and sciatic nerve index (SFI) of the groups were assessed. Results: Time of recovery of sensory parameters after two weeks treatment with NGF significantly decreased when compared to the control group (P<0.01), while the cell therapy groupshowed siqnificant improvement. At the end of the sixth week, mean of SFI in cell therapy and NGF groups increased compared to the control group but the differences were not significant. Mean NCV and AMP also showed a non-significant increase in the cell therapy group compared to the control group. Conclusion: Transplantation of non-autologous of adipose-derived stem cells (ASDc) is an appropriate therapeutic approach in repairing of neurological injuries and NGF has a positive effect in crushed sciatic nerve regeneration.