Vesicle-Associated Membrane Protein-Associated Protein A Is Involved in Androgen Receptor Trafficking in Mouse Sertoli Cells

Vesicle-Associated Membrane Protein-Associated Protein A Is Involved in Androgen Receptor Trafficking in Mouse Sertoli Cells

Androgen and its receptor (AR) play an important role in maintaining spermatogenesis and male fertility. The nonclassical androgen signaling pathway is proposed to be mediated by an AR in plasma membrane in Sertoli cells. Our previous studies showed that testosterone induces cytoplasmic AR transloca...

Full description

Bibliographic Details
Main Authors: Qiong Deng, Jianwen Zhang, Zhu Wang, Shengping Zhang, Fan Zhi, Hui Liang
Format: Article
Language:English
Published: Hindawi Limited 2018-01-01
Series:International Journal of Endocrinology
Online Access:http://dx.doi.org/10.1155/2018/4537214
id doaj-35802b92a5444c82ac1aa9b819e8d40c
record_format Article
spelling doaj-35802b92a5444c82ac1aa9b819e8d40c2020-11-24T21:46:44ZengHindawi LimitedInternational Journal of Endocrinology1687-83371687-83452018-01-01201810.1155/2018/45372144537214Vesicle-Associated Membrane Protein-Associated Protein A Is Involved in Androgen Receptor Trafficking in Mouse Sertoli CellsQiong Deng0Jianwen Zhang1Zhu Wang2Shengping Zhang3Fan Zhi4Hui Liang5Department of Urology, The People’s Hospital of Longhua, Shenzhen, ChinaDepartment of Urology, The People’s Hospital of Longhua, Shenzhen, ChinaDepartment of Urology, The People’s Hospital of Longhua, Shenzhen, ChinaDepartment of Urology, The People’s Hospital of Longhua, Shenzhen, ChinaDepartment of Urology, The People’s Hospital of Longhua, Shenzhen, ChinaDepartment of Urology, The People’s Hospital of Longhua, Shenzhen, ChinaAndrogen and its receptor (AR) play an important role in maintaining spermatogenesis and male fertility. The nonclassical androgen signaling pathway is proposed to be mediated by an AR in plasma membrane in Sertoli cells. Our previous studies showed that testosterone induces cytoplasmic AR translocation to plasma membrane by binding with caveolin-1. This study was conducted to the underlying molecular mechanism mediating AR trafficking. Data from mass spectrometry using membrane coimmunoprecipitation sample by anti-AR antibody indicated VAPA is a candidate protein. Knockdown of VAPA by shRNA decreased the amount of AR localized to membrane and nuclear fraction and prevented AR trafficking after being exposed to testosterone. Further studies indicated AR trafficking in Sertoli cells might be mediated by VAPA via association with vesicle transport protein OSBP. This study can enrich the mechanism of the androgen actions and will be helpful for further clarifying the nonclassical signaling pathway of androgens in Sertoli cells.http://dx.doi.org/10.1155/2018/4537214
collection DOAJ
language English
format Article
sources DOAJ
author Qiong Deng
Jianwen Zhang
Zhu Wang
Shengping Zhang
Fan Zhi
Hui Liang
spellingShingle Qiong Deng
Jianwen Zhang
Zhu Wang
Shengping Zhang
Fan Zhi
Hui Liang
Vesicle-Associated Membrane Protein-Associated Protein A Is Involved in Androgen Receptor Trafficking in Mouse Sertoli Cells
International Journal of Endocrinology
author_facet Qiong Deng
Jianwen Zhang
Zhu Wang
Shengping Zhang
Fan Zhi
Hui Liang
author_sort Qiong Deng
title Vesicle-Associated Membrane Protein-Associated Protein A Is Involved in Androgen Receptor Trafficking in Mouse Sertoli Cells
title_short Vesicle-Associated Membrane Protein-Associated Protein A Is Involved in Androgen Receptor Trafficking in Mouse Sertoli Cells
title_full Vesicle-Associated Membrane Protein-Associated Protein A Is Involved in Androgen Receptor Trafficking in Mouse Sertoli Cells
title_fullStr Vesicle-Associated Membrane Protein-Associated Protein A Is Involved in Androgen Receptor Trafficking in Mouse Sertoli Cells
title_full_unstemmed Vesicle-Associated Membrane Protein-Associated Protein A Is Involved in Androgen Receptor Trafficking in Mouse Sertoli Cells
title_sort vesicle-associated membrane protein-associated protein a is involved in androgen receptor trafficking in mouse sertoli cells
publisher Hindawi Limited
series International Journal of Endocrinology
issn 1687-8337
1687-8345
publishDate 2018-01-01
description Androgen and its receptor (AR) play an important role in maintaining spermatogenesis and male fertility. The nonclassical androgen signaling pathway is proposed to be mediated by an AR in plasma membrane in Sertoli cells. Our previous studies showed that testosterone induces cytoplasmic AR translocation to plasma membrane by binding with caveolin-1. This study was conducted to the underlying molecular mechanism mediating AR trafficking. Data from mass spectrometry using membrane coimmunoprecipitation sample by anti-AR antibody indicated VAPA is a candidate protein. Knockdown of VAPA by shRNA decreased the amount of AR localized to membrane and nuclear fraction and prevented AR trafficking after being exposed to testosterone. Further studies indicated AR trafficking in Sertoli cells might be mediated by VAPA via association with vesicle transport protein OSBP. This study can enrich the mechanism of the androgen actions and will be helpful for further clarifying the nonclassical signaling pathway of androgens in Sertoli cells.
url http://dx.doi.org/10.1155/2018/4537214
work_keys_str_mv AT qiongdeng vesicleassociatedmembraneproteinassociatedproteinaisinvolvedinandrogenreceptortraffickinginmousesertolicells
AT jianwenzhang vesicleassociatedmembraneproteinassociatedproteinaisinvolvedinandrogenreceptortraffickinginmousesertolicells
AT zhuwang vesicleassociatedmembraneproteinassociatedproteinaisinvolvedinandrogenreceptortraffickinginmousesertolicells
AT shengpingzhang vesicleassociatedmembraneproteinassociatedproteinaisinvolvedinandrogenreceptortraffickinginmousesertolicells
AT fanzhi vesicleassociatedmembraneproteinassociatedproteinaisinvolvedinandrogenreceptortraffickinginmousesertolicells
AT huiliang vesicleassociatedmembraneproteinassociatedproteinaisinvolvedinandrogenreceptortraffickinginmousesertolicells
_version_ 1725900315247509504

Similar Items