In vivo monitoring of hepatic glycolipid distribution of <i>n</i>-6&thinsp;∕&thinsp;<i>n</i>-3 in jugular-vein-cannulated rats as a nutritional research model for monogastric animal

• Main Authors: S.-O. Park, V. A. Zammit
• Format: Article
• Language: English
• Published: Copernicus Publications 2019-07-01
• Series: Archives Animal Breeding
• Online Access: https://www.arch-anim-breed.net/62/437/2019/aab-62-437-2019.pdf

<p>The metabolic distribution via blood from liver of glycerolipids by omega-6 to omega-3 fatty acid (<span class="inline-formula"><i>n</i></span>-6&thinsp;<span class="inline-formula">∕</span>&thinsp;<span class="inline-formula"><i>n</i></span>-3) ratio in monogastric animal nutrition is very important. In vivo monitoring technique using jugular-vein-cannulated rats as a nutritional model for monogastric animal can yield important insights into animal nutrition. This study was conducted to determine the effect of different <span class="inline-formula"><i>n</i></span>-6&thinsp;<span class="inline-formula">∕</span>&thinsp;<span class="inline-formula"><i>n</i></span>-3 ratios (<span class="inline-formula">71:1</span>, <span class="inline-formula">4:1</span>, <span class="inline-formula">15:1</span>, <span class="inline-formula">30:1</span>) on metabolic distribution of glycerolipids newly synthesized and secreted in the liver of the rats and explore the mechanism involved. Regarding <span class="inline-formula">¹⁴CO₂</span> released from oxidation of glycerolipid metabolism, it was the highest (22.5&thinsp;%) in groups with a <span class="inline-formula"><i>n</i></span>-6&thinsp;<span class="inline-formula">∕</span>&thinsp;<span class="inline-formula"><i>n</i></span>-3 ratio of <span class="inline-formula">4:1</span> (<span class="inline-formula"><i>P</i>&lt;0.05</span>). The control group showed the highest total glycerolipid level, followed by the <span class="inline-formula">30:1</span>, <span class="inline-formula">15:1</span>, and <span class="inline-formula">4:1</span> groups in order (<span class="inline-formula"><i>P</i>&lt;0.05</span>). When secreted triacylglycerol level of each group was compared with that of the control group, the <span class="inline-formula">4:1</span>, <span class="inline-formula">15:1</span>, and <span class="inline-formula">30:1</span> groups were decreased by 36.3&thinsp;%, 20.9&thinsp;%, and 13.3&thinsp;%, respectively (<span class="inline-formula"><i>P</i>&lt;0.05</span>). Regarding the distribution of phospholipid against total glycerolipid compared to the control group, the <span class="inline-formula">4:1</span>, <span class="inline-formula">15:1</span>, and <span class="inline-formula">30:1</span> groups were 1.38, 1.29, and 1.17 times higher, respectively (<span class="inline-formula"><i>P</i>&lt;0.05</span>). In the comparison of <span class="inline-formula">¹⁴CO₂</span> emission against total glycerolipid compared with the control group, the <span class="inline-formula">4:1</span>, <span class="inline-formula">15:1</span>, and <span class="inline-formula">30:1</span> groups were 1.61, 1.52, and 1.29 times higher, respectively (<span class="inline-formula"><i>P</i>&lt;0.05</span>). These results demonstrate that a dietary <span class="inline-formula"><i>n</i></span>-6&thinsp;<span class="inline-formula">∕</span>&thinsp;<span class="inline-formula"><i>n</i></span>-3 fatty acid ratio of <span class="inline-formula">4:1</span> could significantly decrease harmful lipid levels in the blood by controlling the mechanism of metabolic distribution via blood from triglyceride and phospholipid newly synthesized in the liver of cannulated rat.</p>