Capacity of rTth polymerase to detect RNA in the presence of various inhibitors.
The full potential of the real-time reverse transcription polymerase chain reaction (RT-PCR) as a rapid and accurate diagnostic method is limited by DNA polymerase inhibitors as well as reverse transcriptase inhibitors which are ubiquitous in clinical samples. rTth polymerase has proven to be more r...
Main Authors: | Dongyang Cai, Ole Behrmann, Frank Hufert, Gregory Dame, Gerald Urban |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2018-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC5749758?pdf=render |
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