Effects of Astaxanthin from Shrimp Shell on Oxidative Stress and Behavior in Animal Model of Alzheimer’s Disease
This study aimed to investigate the effect of astaxanthin (ASX) extracted and ASX powder from shrimp (<i>Litopenaeus vannamei</i>) shells on Wistar rats with Alzheimer’s disease, induced by amyloid-β (1-42) peptides. In this task, the rats were divided into eight group...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-11-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/17/11/628 |
| id |
doaj-3509b11443fb4786aff61257bf041a9e |
|---|---|
| record_format |
Article |
| spelling |
doaj-3509b11443fb4786aff61257bf041a9e2020-11-24T21:33:51ZengMDPI AGMarine Drugs1660-33972019-11-01171162810.3390/md17110628md17110628Effects of Astaxanthin from Shrimp Shell on Oxidative Stress and Behavior in Animal Model of Alzheimer’s DiseaseTakunrat Taksima0Pennapa Chonpathompikunlert1Morakot Sroyraya2Pilaiwanwadee Hutamekalin3Maruj Limpawattana4Wanwimol Klaypradit5Department of Fishery Products, Faculty of Fisheries, Kasetsart University, Bangkok 10900, ThailandExpert Centre of Innovative Health Food (InnoFood) Thailand Institute of Scientific and Technological Research (TISTR) 35 Moo 3 Technopolis, Khlong 5, Khlong Luang, Pathum Thani 12120, ThailandDepartment of Anatomy, Faculty of Science, Mahidol University, Bangkok 10400, ThailandDepartment of Physiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, ThailandDepartment of Food Technology, Faculty of Science, Siam University, Bangkok 10160, ThailandDepartment of Fishery Products, Faculty of Fisheries, Kasetsart University, Bangkok 10900, ThailandThis study aimed to investigate the effect of astaxanthin (ASX) extracted and ASX powder from shrimp (<i>Litopenaeus vannamei</i>) shells on Wistar rats with Alzheimer’s disease, induced by amyloid-β (1-42) peptides. In this task, the rats were divided into eight groups: (1) Control, (2) sham operate, (3) negative control (vehicle) + Aβ<sub>1-42</sub>, (4) ASX extract+Aβ<sub>1-42</sub>, (5) commercial ASX + Aβ<sub>1-42</sub>, (6) ASX powder + Aβ<sub>1-42</sub>, (7) blank powder + Aβ<sub>1-42</sub>, and (8) vitamin E + Aβ<sub>1-42</sub>. All treatments were orally administrated for 30 days. At 14- and 29-days post injection, animals were observed in behavioral tests. On the 31st day, animals were sacrificed; the hippocampus and cortex were collected. Those two brain areas were then homogenized and stored for biochemical and histological analysis. The results showed that the Aβ<sub>1-42</sub> infused group significantly reduced cognitive ability and increased memory loss, as assessed by the Morris water maze test, novel object recognition test, and novel object location test. Moreover, the Aβ<sub>1-42</sub> infused group exhibited a deterioration of oxidative markers, including glutathione peroxidase enzymes (GPx), lipid peroxidation (MDA), products of protein oxidation, and superoxide anion in the cortex and the hippocampus. Meanwhile, ASX powder (10 mg/kg body weight) showed a significant reduction in cognitive and memory impairments and oxidative stress which is greater than ASX extract in the same dose of compound or vitamin E (100 mg/kg body weight). Our study indicates the beneficial properties of ASX in alleviation of cognitive functions and reducing neurodegeneration in Wistar rats induced by amyloid-β (1-42) peptides.https://www.mdpi.com/1660-3397/17/11/628astaxanthinalzheimer’s diseaseamyloid-β (1-42) peptidesencapsulationshrimp shells |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Takunrat Taksima Pennapa Chonpathompikunlert Morakot Sroyraya Pilaiwanwadee Hutamekalin Maruj Limpawattana Wanwimol Klaypradit |
| spellingShingle |
Takunrat Taksima Pennapa Chonpathompikunlert Morakot Sroyraya Pilaiwanwadee Hutamekalin Maruj Limpawattana Wanwimol Klaypradit Effects of Astaxanthin from Shrimp Shell on Oxidative Stress and Behavior in Animal Model of Alzheimer’s Disease Marine Drugs astaxanthin alzheimer’s disease amyloid-β (1-42) peptides encapsulation shrimp shells |
| author_facet |
Takunrat Taksima Pennapa Chonpathompikunlert Morakot Sroyraya Pilaiwanwadee Hutamekalin Maruj Limpawattana Wanwimol Klaypradit |
| author_sort |
Takunrat Taksima |
| title |
Effects of Astaxanthin from Shrimp Shell on Oxidative Stress and Behavior in Animal Model of Alzheimer’s Disease |
| title_short |
Effects of Astaxanthin from Shrimp Shell on Oxidative Stress and Behavior in Animal Model of Alzheimer’s Disease |
| title_full |
Effects of Astaxanthin from Shrimp Shell on Oxidative Stress and Behavior in Animal Model of Alzheimer’s Disease |
| title_fullStr |
Effects of Astaxanthin from Shrimp Shell on Oxidative Stress and Behavior in Animal Model of Alzheimer’s Disease |
| title_full_unstemmed |
Effects of Astaxanthin from Shrimp Shell on Oxidative Stress and Behavior in Animal Model of Alzheimer’s Disease |
| title_sort |
effects of astaxanthin from shrimp shell on oxidative stress and behavior in animal model of alzheimer’s disease |
| publisher |
MDPI AG |
| series |
Marine Drugs |
| issn |
1660-3397 |
| publishDate |
2019-11-01 |
| description |
This study aimed to investigate the effect of astaxanthin (ASX) extracted and ASX powder from shrimp (<i>Litopenaeus vannamei</i>) shells on Wistar rats with Alzheimer’s disease, induced by amyloid-β (1-42) peptides. In this task, the rats were divided into eight groups: (1) Control, (2) sham operate, (3) negative control (vehicle) + Aβ<sub>1-42</sub>, (4) ASX extract+Aβ<sub>1-42</sub>, (5) commercial ASX + Aβ<sub>1-42</sub>, (6) ASX powder + Aβ<sub>1-42</sub>, (7) blank powder + Aβ<sub>1-42</sub>, and (8) vitamin E + Aβ<sub>1-42</sub>. All treatments were orally administrated for 30 days. At 14- and 29-days post injection, animals were observed in behavioral tests. On the 31st day, animals were sacrificed; the hippocampus and cortex were collected. Those two brain areas were then homogenized and stored for biochemical and histological analysis. The results showed that the Aβ<sub>1-42</sub> infused group significantly reduced cognitive ability and increased memory loss, as assessed by the Morris water maze test, novel object recognition test, and novel object location test. Moreover, the Aβ<sub>1-42</sub> infused group exhibited a deterioration of oxidative markers, including glutathione peroxidase enzymes (GPx), lipid peroxidation (MDA), products of protein oxidation, and superoxide anion in the cortex and the hippocampus. Meanwhile, ASX powder (10 mg/kg body weight) showed a significant reduction in cognitive and memory impairments and oxidative stress which is greater than ASX extract in the same dose of compound or vitamin E (100 mg/kg body weight). Our study indicates the beneficial properties of ASX in alleviation of cognitive functions and reducing neurodegeneration in Wistar rats induced by amyloid-β (1-42) peptides. |
| topic |
astaxanthin alzheimer’s disease amyloid-β (1-42) peptides encapsulation shrimp shells |
| url |
https://www.mdpi.com/1660-3397/17/11/628 |
| work_keys_str_mv |
AT takunrattaksima effectsofastaxanthinfromshrimpshellonoxidativestressandbehaviorinanimalmodelofalzheimersdisease AT pennapachonpathompikunlert effectsofastaxanthinfromshrimpshellonoxidativestressandbehaviorinanimalmodelofalzheimersdisease AT morakotsroyraya effectsofastaxanthinfromshrimpshellonoxidativestressandbehaviorinanimalmodelofalzheimersdisease AT pilaiwanwadeehutamekalin effectsofastaxanthinfromshrimpshellonoxidativestressandbehaviorinanimalmodelofalzheimersdisease AT marujlimpawattana effectsofastaxanthinfromshrimpshellonoxidativestressandbehaviorinanimalmodelofalzheimersdisease AT wanwimolklaypradit effectsofastaxanthinfromshrimpshellonoxidativestressandbehaviorinanimalmodelofalzheimersdisease |
| _version_ |
1725951642702970880 |