An Improved Scalable Hydrogel Dish for Spheroid Culture

Research in fields studying cellular response to surface tension and mechanical forces necessitate cell culture tools with tunability of substrate stiffness. We created a scalable hydrogel dish design to facilitate scaffold-free formation of multiple spheroids in a single dish. Our novel design feat...

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Main Authors:	Jonard Corpuz Valdoz, Dallin J. Jacobs, Collin G. Cribbs, Benjamin C. Johnson, Brandon M. Hemeyer, Ethan L. Dodson, Jordan A. Saunooke, Nicholas A. Franks, Peter Daniel Poulson, Seth R. Garfield, Connor J. Knight, Pam M. Van Ry
Format:	Article
Language:	English
Published:	MDPI AG 2021-06-01
Series:	Life
Subjects:	3D culture hydrogel spheroid primary-derived spheroid ultra-low adherence scaffold-free
Online Access:	https://www.mdpi.com/2075-1729/11/6/517

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spelling	doaj-34ede55eb8ba47be8ea258ac5c016f812021-06-30T23:11:38ZengMDPI AGLife2075-17292021-06-011151751710.3390/life11060517An Improved Scalable Hydrogel Dish for Spheroid CultureJonard Corpuz Valdoz0Dallin J. Jacobs1Collin G. Cribbs2Benjamin C. Johnson3Brandon M. Hemeyer4Ethan L. Dodson5Jordan A. Saunooke6Nicholas A. Franks7Peter Daniel Poulson8Seth R. Garfield9Connor J. Knight10Pam M. Van Ry11Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USADepartment of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USADepartment of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USADepartment of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USADepartment of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USADepartment of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USADepartment of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USADepartment of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USADepartment of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USADepartment of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USADepartment of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USADepartment of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USAResearch in fields studying cellular response to surface tension and mechanical forces necessitate cell culture tools with tunability of substrate stiffness. We created a scalable hydrogel dish design to facilitate scaffold-free formation of multiple spheroids in a single dish. Our novel design features inner and outer walls, allowing efficient media changes and downstream experiments. The design is easily scalable, accommodating varying numbers of microwells per plate. We report that non-adherent hydrogel stiffness affects spheroid morphology and compaction. We found that spheroid morphology and viability in our hydrogel dishes were comparable to commercially available Aggrewell™800 plates, with improved tunability of surface stiffness and imaging area. Device function was demonstrated with a migration assay using two investigational inhibitors against EMT. We successfully maintained primary-derived spheroids from murine and porcine lungs in the hydrogel dish. These features increase the ability to produce highly consistent cell aggregates for biological research.https://www.mdpi.com/2075-1729/11/6/5173D culturehydrogelspheroidprimary-derived spheroidultra-low adherencescaffold-free
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Jonard Corpuz Valdoz Dallin J. Jacobs Collin G. Cribbs Benjamin C. Johnson Brandon M. Hemeyer Ethan L. Dodson Jordan A. Saunooke Nicholas A. Franks Peter Daniel Poulson Seth R. Garfield Connor J. Knight Pam M. Van Ry
spellingShingle	Jonard Corpuz Valdoz Dallin J. Jacobs Collin G. Cribbs Benjamin C. Johnson Brandon M. Hemeyer Ethan L. Dodson Jordan A. Saunooke Nicholas A. Franks Peter Daniel Poulson Seth R. Garfield Connor J. Knight Pam M. Van Ry An Improved Scalable Hydrogel Dish for Spheroid Culture Life 3D culture hydrogel spheroid primary-derived spheroid ultra-low adherence scaffold-free
author_facet	Jonard Corpuz Valdoz Dallin J. Jacobs Collin G. Cribbs Benjamin C. Johnson Brandon M. Hemeyer Ethan L. Dodson Jordan A. Saunooke Nicholas A. Franks Peter Daniel Poulson Seth R. Garfield Connor J. Knight Pam M. Van Ry
author_sort	Jonard Corpuz Valdoz
title	An Improved Scalable Hydrogel Dish for Spheroid Culture
title_short	An Improved Scalable Hydrogel Dish for Spheroid Culture
title_full	An Improved Scalable Hydrogel Dish for Spheroid Culture
title_fullStr	An Improved Scalable Hydrogel Dish for Spheroid Culture
title_full_unstemmed	An Improved Scalable Hydrogel Dish for Spheroid Culture
title_sort	improved scalable hydrogel dish for spheroid culture
publisher	MDPI AG
series	Life
issn	2075-1729
publishDate	2021-06-01
description	Research in fields studying cellular response to surface tension and mechanical forces necessitate cell culture tools with tunability of substrate stiffness. We created a scalable hydrogel dish design to facilitate scaffold-free formation of multiple spheroids in a single dish. Our novel design features inner and outer walls, allowing efficient media changes and downstream experiments. The design is easily scalable, accommodating varying numbers of microwells per plate. We report that non-adherent hydrogel stiffness affects spheroid morphology and compaction. We found that spheroid morphology and viability in our hydrogel dishes were comparable to commercially available Aggrewell™800 plates, with improved tunability of surface stiffness and imaging area. Device function was demonstrated with a migration assay using two investigational inhibitors against EMT. We successfully maintained primary-derived spheroids from murine and porcine lungs in the hydrogel dish. These features increase the ability to produce highly consistent cell aggregates for biological research.
topic	3D culture hydrogel spheroid primary-derived spheroid ultra-low adherence scaffold-free
url	https://www.mdpi.com/2075-1729/11/6/517
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An Improved Scalable Hydrogel Dish for Spheroid Culture

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