A disposable DNA methylation sensor based on the printable graphene field effect transistor

• Main Authors: Jia Yunfang, Zhang Jizhao, Fan Qingjie
• Format: Article
• Language: English
• Published: EDP Sciences 2021-01-01
• Series: E3S Web of Conferences
• Online Access: https://www.e3s-conferences.org/articles/e3sconf/pdf/2021/47/e3sconf_icepe2021_04045.pdf

The detection of DNA methylation is necessary for the research of epigenetics. In this work we would like to propose a disposable DNA methylation sensor by using graphene field effect transistor (GFET) as the sensing platform. In this component, the liquid-phase exfoliated graphene (LEG) nanosheets were drop-coated on the flexible substrates of polyethylene terephthalate (PET) films. Then, the interdigital structured electrodes (named as source and drain) were printed on the LEG coated PET films to form the expected GFETs. Thirdly, the carbon dots (CDs) decoration was conducted and examined on the asprepared GFETs to evaluate the influence of CDs, as well as optimize CDs’ concentration. At last, the immune identification-based sensing strategy was utilized on the CDs modified GFETs to develop the concerned DNA methylation sensor. The experimental data indicate the proposed sensors could be a potential experimental tool for epigenetic research.