The Combination of Stromal Vascular Fraction Cells and Platelet-Rich Plasma Reduces Malondialdehyde and Nitric Oxide Levels in Deep Dermal Burn Injury

• Main Authors: Josh F, Soekamto TH, Adriani JR, Jonatan B, Mizuno H, Faruk M
• Format: Article
• Language: English
• Published: Dove Medical Press 2021-07-01
• Series: Journal of Inflammation Research
• Subjects: stromal vascular fraction cells; platelet rich plasma; malondialdehyde; nitric oxide; burn injury
• Online Access: https://www.dovepress.com/the-combination-of-stromal-vascular-fraction-cells-and-platelet-rich-p-peer-reviewed-fulltext-article-JIR
• ISSN: 1178-7031

Fonny Josh,1,2 Tomie Hermawan Soekamto,3 Januar Rizky Adriani,4 Billy Jonatan,4 Hiroshi Mizuno,5 Muhammad Faruk4 1Division of Plastic and Reconstructive Surgery, Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, South Sulawesi, Indonesia; 2Department of Plastic and Reconstructive Surgery, Wahidin Sudirohusodo Hospital, Makassar, South Sulawesi, Indonesia; 3Department of Plastic and Reconstructive Surgery, Koja Hospital, Jakarta, Indonesia; 4Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, South Sulawesi, Indonesia; 5Department of Plastic and Reconstructive Surgery, Juntendo University, Tokyo, JapanCorrespondence: Fonny JoshDivision of Plastic and Reconstructive Surgery, Department of Surgery, Faculty of Medicine, Hasanuddin University, Jalan Perintis Kemerdekaan KM 11, Makassar, 90245, IndonesiaFax [phone]Email [e-mail]: Thermal burns release reactive oxygen species, which cause profound systemic and local changes. Stromal vascular fraction cells (SVFs) combined with platelet-rich plasma accelerate burn wound healing. This study investigated the effect of a combination of locally injected SVFs and PRP on malondialdehyde (MDA) and nitric oxide (NO) serum and tissue levels in a deep dermal burn model in Wistar rats.Methods: Thirty-six adult Wistar rats weighing between 150 and 250 grams were used in this study to establish a deep dermal degree burn wound model. They were randomly divided into 4 groups: locally injected the combination SVFs and PRP, the Vaseline group, the placebo group, and healthy Wistar rats (the normal control group). MDA and NO levels in blood serum and burn wound tissue were measured at 8, 24, and 48 hours. Data were analyzed with one-way ANOVA followed by multiple comparisons tests and regression tests.Results: Local injection of SVFs and PRP in combination affected blood MDA, tissue MDA, blood NO and tissue NO levels, with reductions of 0.257μmol/L, 0.427 μmol/L, 21.78nmol/mg, and 23.777nmol/mg, respectively. Injection of SVFs and PRP in combination reduced tissue MDA levels by 1.282 times, NO blood levels by 2.305, and NO tissue levels by 2.377 times compared to Vaseline application.Conclusion: The combination of SVFs and PRP undeniably reduced the MDA and NO levels in blood and tissue compared to those in the Vaseline and placebo groups. The injection of these two preparations in combination inhibited the local and systemic stress oxidative response, as illustrated by the decreased MDA and NO levels in blood serum and tissue.Keywords: stromal vascular fraction cells, platelet rich plasma, malondialdehyde, nitric oxide, burn injury