Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates
Abstract Brucella canis infection is an underdiagnosed zoonotic disease. Knowledge about perinatal brucellosis in dogs is extremely limited, although foetuses and neonates are under risk of infection due to vertical transmission. In this study, immunohistochemistry was used to determine tissue distr...
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doaj-349ea1a0071d40828628eaa2a49dac692020-12-08T06:16:00ZengNature Publishing GroupScientific Reports2045-23222018-05-018111010.1038/s41598-018-25651-xTissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonatesTayse Domingues de Souza0Tatiane Furtado de Carvalho1Juliana Pinto da Silva Mol2João Vítor Menezes Lopes3Monique Ferreira Silva4Tatiane Alves da Paixão5Renato Lima Santos6Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas GeraisDepartamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas GeraisDepartamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas GeraisCurso de Medicina Veterinária, Universidade Vila VelhaDepartamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas GeraisDepartamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas GeraisDepartamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas GeraisAbstract Brucella canis infection is an underdiagnosed zoonotic disease. Knowledge about perinatal brucellosis in dogs is extremely limited, although foetuses and neonates are under risk of infection due to vertical transmission. In this study, immunohistochemistry was used to determine tissue distribution and cell tropism of B. canis in canine foetuses and neonates. Diagnosis of B. canis in tissues of naturally infected pups was based on PCR and sequencing of amplicons, bacterial isolation, and immunohistochemistry, whose specificity was confirmed by laser capture microdissection. PCR positivity among 200 puppies was 21%, and nine isolates of B. canis were obtained. Tissues from 13 PCR-positive puppies (4 stillborn and 9 neonates) presented widespread immunolabeling. Stomach, intestines, kidney, nervous system, and umbilicus were positive in all animals tested. Other frequently infected organs included the liver (92%), lungs (85%), lymph nodes (69%), and spleen (62%). Immunolabeled coccobacilli occurred mostly in macrophages, but they were also observed in erythrocytes, epithelial cells of gastrointestinal mucosa, renal tubules, epidermis, adipocytes, choroid plexus, ependyma, neuroblasts, blood vessels endothelium, muscle cells, and in the intestinal lumen. These results largely expand our knowledge about perinatal brucellosis in the dog, clearly demonstrating a pantropic distribution of B. canis in naturally infected foetuses and neonates.https://doi.org/10.1038/s41598-018-25651-x |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tayse Domingues de Souza Tatiane Furtado de Carvalho Juliana Pinto da Silva Mol João Vítor Menezes Lopes Monique Ferreira Silva Tatiane Alves da Paixão Renato Lima Santos |
spellingShingle |
Tayse Domingues de Souza Tatiane Furtado de Carvalho Juliana Pinto da Silva Mol João Vítor Menezes Lopes Monique Ferreira Silva Tatiane Alves da Paixão Renato Lima Santos Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates Scientific Reports |
author_facet |
Tayse Domingues de Souza Tatiane Furtado de Carvalho Juliana Pinto da Silva Mol João Vítor Menezes Lopes Monique Ferreira Silva Tatiane Alves da Paixão Renato Lima Santos |
author_sort |
Tayse Domingues de Souza |
title |
Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates |
title_short |
Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates |
title_full |
Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates |
title_fullStr |
Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates |
title_full_unstemmed |
Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates |
title_sort |
tissue distribution and cell tropism of brucella canis in naturally infected canine foetuses and neonates |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2018-05-01 |
description |
Abstract Brucella canis infection is an underdiagnosed zoonotic disease. Knowledge about perinatal brucellosis in dogs is extremely limited, although foetuses and neonates are under risk of infection due to vertical transmission. In this study, immunohistochemistry was used to determine tissue distribution and cell tropism of B. canis in canine foetuses and neonates. Diagnosis of B. canis in tissues of naturally infected pups was based on PCR and sequencing of amplicons, bacterial isolation, and immunohistochemistry, whose specificity was confirmed by laser capture microdissection. PCR positivity among 200 puppies was 21%, and nine isolates of B. canis were obtained. Tissues from 13 PCR-positive puppies (4 stillborn and 9 neonates) presented widespread immunolabeling. Stomach, intestines, kidney, nervous system, and umbilicus were positive in all animals tested. Other frequently infected organs included the liver (92%), lungs (85%), lymph nodes (69%), and spleen (62%). Immunolabeled coccobacilli occurred mostly in macrophages, but they were also observed in erythrocytes, epithelial cells of gastrointestinal mucosa, renal tubules, epidermis, adipocytes, choroid plexus, ependyma, neuroblasts, blood vessels endothelium, muscle cells, and in the intestinal lumen. These results largely expand our knowledge about perinatal brucellosis in the dog, clearly demonstrating a pantropic distribution of B. canis in naturally infected foetuses and neonates. |
url |
https://doi.org/10.1038/s41598-018-25651-x |
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