Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates

Abstract Brucella canis infection is an underdiagnosed zoonotic disease. Knowledge about perinatal brucellosis in dogs is extremely limited, although foetuses and neonates are under risk of infection due to vertical transmission. In this study, immunohistochemistry was used to determine tissue distr...

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Main Authors: Tayse Domingues de Souza, Tatiane Furtado de Carvalho, Juliana Pinto da Silva Mol, João Vítor Menezes Lopes, Monique Ferreira Silva, Tatiane Alves da Paixão, Renato Lima Santos
Format: Article
Language:English
Published: Nature Publishing Group 2018-05-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-018-25651-x
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spelling doaj-349ea1a0071d40828628eaa2a49dac692020-12-08T06:16:00ZengNature Publishing GroupScientific Reports2045-23222018-05-018111010.1038/s41598-018-25651-xTissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonatesTayse Domingues de Souza0Tatiane Furtado de Carvalho1Juliana Pinto da Silva Mol2João Vítor Menezes Lopes3Monique Ferreira Silva4Tatiane Alves da Paixão5Renato Lima Santos6Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas GeraisDepartamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas GeraisDepartamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas GeraisCurso de Medicina Veterinária, Universidade Vila VelhaDepartamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas GeraisDepartamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas GeraisDepartamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas GeraisAbstract Brucella canis infection is an underdiagnosed zoonotic disease. Knowledge about perinatal brucellosis in dogs is extremely limited, although foetuses and neonates are under risk of infection due to vertical transmission. In this study, immunohistochemistry was used to determine tissue distribution and cell tropism of B. canis in canine foetuses and neonates. Diagnosis of B. canis in tissues of naturally infected pups was based on PCR and sequencing of amplicons, bacterial isolation, and immunohistochemistry, whose specificity was confirmed by laser capture microdissection. PCR positivity among 200 puppies was 21%, and nine isolates of B. canis were obtained. Tissues from 13 PCR-positive puppies (4 stillborn and 9 neonates) presented widespread immunolabeling. Stomach, intestines, kidney, nervous system, and umbilicus were positive in all animals tested. Other frequently infected organs included the liver (92%), lungs (85%), lymph nodes (69%), and spleen (62%). Immunolabeled coccobacilli occurred mostly in macrophages, but they were also observed in erythrocytes, epithelial cells of gastrointestinal mucosa, renal tubules, epidermis, adipocytes, choroid plexus, ependyma, neuroblasts, blood vessels endothelium, muscle cells, and in the intestinal lumen. These results largely expand our knowledge about perinatal brucellosis in the dog, clearly demonstrating a pantropic distribution of B. canis in naturally infected foetuses and neonates.https://doi.org/10.1038/s41598-018-25651-x
collection DOAJ
language English
format Article
sources DOAJ
author Tayse Domingues de Souza
Tatiane Furtado de Carvalho
Juliana Pinto da Silva Mol
João Vítor Menezes Lopes
Monique Ferreira Silva
Tatiane Alves da Paixão
Renato Lima Santos
spellingShingle Tayse Domingues de Souza
Tatiane Furtado de Carvalho
Juliana Pinto da Silva Mol
João Vítor Menezes Lopes
Monique Ferreira Silva
Tatiane Alves da Paixão
Renato Lima Santos
Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates
Scientific Reports
author_facet Tayse Domingues de Souza
Tatiane Furtado de Carvalho
Juliana Pinto da Silva Mol
João Vítor Menezes Lopes
Monique Ferreira Silva
Tatiane Alves da Paixão
Renato Lima Santos
author_sort Tayse Domingues de Souza
title Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates
title_short Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates
title_full Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates
title_fullStr Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates
title_full_unstemmed Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates
title_sort tissue distribution and cell tropism of brucella canis in naturally infected canine foetuses and neonates
publisher Nature Publishing Group
series Scientific Reports
issn 2045-2322
publishDate 2018-05-01
description Abstract Brucella canis infection is an underdiagnosed zoonotic disease. Knowledge about perinatal brucellosis in dogs is extremely limited, although foetuses and neonates are under risk of infection due to vertical transmission. In this study, immunohistochemistry was used to determine tissue distribution and cell tropism of B. canis in canine foetuses and neonates. Diagnosis of B. canis in tissues of naturally infected pups was based on PCR and sequencing of amplicons, bacterial isolation, and immunohistochemistry, whose specificity was confirmed by laser capture microdissection. PCR positivity among 200 puppies was 21%, and nine isolates of B. canis were obtained. Tissues from 13 PCR-positive puppies (4 stillborn and 9 neonates) presented widespread immunolabeling. Stomach, intestines, kidney, nervous system, and umbilicus were positive in all animals tested. Other frequently infected organs included the liver (92%), lungs (85%), lymph nodes (69%), and spleen (62%). Immunolabeled coccobacilli occurred mostly in macrophages, but they were also observed in erythrocytes, epithelial cells of gastrointestinal mucosa, renal tubules, epidermis, adipocytes, choroid plexus, ependyma, neuroblasts, blood vessels endothelium, muscle cells, and in the intestinal lumen. These results largely expand our knowledge about perinatal brucellosis in the dog, clearly demonstrating a pantropic distribution of B. canis in naturally infected foetuses and neonates.
url https://doi.org/10.1038/s41598-018-25651-x
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