Rapid transient expression of human granulocyte-macrophage colony-stimulating factor in two industrial cultivars of tobacco (Nicotiana tabacum L.) by agroinfiltration

Rapid transient expression of human granulocyte-macrophage colony-stimulating factor in two industrial cultivars of tobacco (Nicotiana tabacum L.) by agroinfiltration

We report the production of hGM-CSF cytokine in leaves of industrial tobacco cultivars DH-17 and DH-27 by using Agrobacterium-mediated transient expression. We prove the concept that very high biomass industrial tobacco plants are suitable platforms for rapid, low cost production of foreign proteins...

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Bibliographic Details
Main Authors: Lea Vojta, Lana Ljuma-Skupnjak, Ankica Budimir, Slobodan Vukičević, Hrvoje Fulgosi
Format: Article
Language:English
Published: Elsevier 2015-09-01
Series:Biotechnology Reports
Subjects:
Tobacco
Transient expression
GM-CSF
Vacuum infiltration
Heterologous expression
Online Access:http://www.sciencedirect.com/science/article/pii/S2215017X15000338
Description
Summary:We report the production of hGM-CSF cytokine in leaves of industrial tobacco cultivars DH-17 and DH-27 by using Agrobacterium-mediated transient expression. We prove the concept that very high biomass industrial tobacco plants are suitable platforms for rapid, low cost production of foreign proteins. Successful transient expression of the GM-CSF was achieved in less than three months, opening the possibility for future applications of this approach in rapid response production of various proteins of non-plant origin in industrial tobacco.
ISSN:2215-017X

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