Diversity and Distribution of Phenol Oxidase Producing Fungi From Soda Lake and Description of Curvularia lonarensis sp. nov.

Soda lake is hyper alkaline and saline habitat located in closed craters with high evaporation rate. In current study fungal diversity from water and sediment samples of a soda lake (Lonar lake) located in Buldhana district of Maharashtra, India was investigated using extensive culturomics approach...

Full description

Bibliographic Details
Main Authors: Rahul Sharma, Om Prakash, Mahesh S. Sonawane, Yogesh Nimonkar, Priyanka Gollelu, Rohit Sharma
Format: Article
Language:English
Published: Frontiers Media S.A. 2016-11-01
Series:Frontiers in Microbiology
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01847/full
id doaj-3450d2a5608141269d892052cda230b1
record_format Article
spelling doaj-3450d2a5608141269d892052cda230b12020-11-24T22:54:35ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-11-01710.3389/fmicb.2016.01847226676Diversity and Distribution of Phenol Oxidase Producing Fungi From Soda Lake and Description of Curvularia lonarensis sp. nov.Rahul Sharma0Om Prakash1Mahesh S. Sonawane2Yogesh Nimonkar3Priyanka Gollelu4Rohit Sharma5NATIONAL CENTER FOR CELL SCIENCENATIONAL CENTER FOR CELL SCIENCENATIONAL CENTER FOR CELL SCIENCENATIONAL CENTER FOR CELL SCIENCES.P. UNIVERSITYNATIONAL CENTER FOR CELL SCIENCESoda lake is hyper alkaline and saline habitat located in closed craters with high evaporation rate. In current study fungal diversity from water and sediment samples of a soda lake (Lonar lake) located in Buldhana district of Maharashtra, India was investigated using extensive culturomics approach and mimicking the natural conditions of Lonar lake in culture media. A total of 104 diverse isolates of extremophilic fungi were recovered from this study and phylogenetically characterized by internal transcribed spacer (ITS) region sequencing. In addition, due to important role of phenol oxidase and peroxidase in degradation of toxic phenol, lignin, etc., all isolated pure cultures were also screened for extracellular phenol oxidase and peroxidase production potential. Diversity analysis indicated that different groups of extremophilic fungi are present in the water and sediment samples of Lonar lake. A total of 38 species of fungi belonging to 18-different genera were recovered. Out of 104 isolates 32 showed ≤ 97% sequences similarity, which were morphologically different and could be potential novel isolates of extremophilic fungi. However, out of 104 isolates only 14 showed the extracellular phenol oxidase production potentials at alkaline pH. Curvularia sp. strain MEF018 showed highest phenol oxidase production at alkaline condition and had low sequence similarity with previously characterized species (96% with Curvularia pseudorobusta). Taxonomic characterization (morphological and physiological) and multi locus sequence analysis (MLSA) using combined alignment of ITS-LSU-gpd of strain MEF018 showed that it is a novel species of the genus Curvularia and hence proposed as Curvularia lonarensis sp. nov.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01847/fullphenol oxidaseFungal diversityCurvulariaextremophilic fungiLonar- lakeSoda-lake
collection DOAJ
language English
format Article
sources DOAJ
author Rahul Sharma
Om Prakash
Mahesh S. Sonawane
Yogesh Nimonkar
Priyanka Gollelu
Rohit Sharma
spellingShingle Rahul Sharma
Om Prakash
Mahesh S. Sonawane
Yogesh Nimonkar
Priyanka Gollelu
Rohit Sharma
Diversity and Distribution of Phenol Oxidase Producing Fungi From Soda Lake and Description of Curvularia lonarensis sp. nov.
Frontiers in Microbiology
phenol oxidase
Fungal diversity
Curvularia
extremophilic fungi
Lonar- lake
Soda-lake
author_facet Rahul Sharma
Om Prakash
Mahesh S. Sonawane
Yogesh Nimonkar
Priyanka Gollelu
Rohit Sharma
author_sort Rahul Sharma
title Diversity and Distribution of Phenol Oxidase Producing Fungi From Soda Lake and Description of Curvularia lonarensis sp. nov.
title_short Diversity and Distribution of Phenol Oxidase Producing Fungi From Soda Lake and Description of Curvularia lonarensis sp. nov.
title_full Diversity and Distribution of Phenol Oxidase Producing Fungi From Soda Lake and Description of Curvularia lonarensis sp. nov.
title_fullStr Diversity and Distribution of Phenol Oxidase Producing Fungi From Soda Lake and Description of Curvularia lonarensis sp. nov.
title_full_unstemmed Diversity and Distribution of Phenol Oxidase Producing Fungi From Soda Lake and Description of Curvularia lonarensis sp. nov.
title_sort diversity and distribution of phenol oxidase producing fungi from soda lake and description of curvularia lonarensis sp. nov.
publisher Frontiers Media S.A.
series Frontiers in Microbiology
issn 1664-302X
publishDate 2016-11-01
description Soda lake is hyper alkaline and saline habitat located in closed craters with high evaporation rate. In current study fungal diversity from water and sediment samples of a soda lake (Lonar lake) located in Buldhana district of Maharashtra, India was investigated using extensive culturomics approach and mimicking the natural conditions of Lonar lake in culture media. A total of 104 diverse isolates of extremophilic fungi were recovered from this study and phylogenetically characterized by internal transcribed spacer (ITS) region sequencing. In addition, due to important role of phenol oxidase and peroxidase in degradation of toxic phenol, lignin, etc., all isolated pure cultures were also screened for extracellular phenol oxidase and peroxidase production potential. Diversity analysis indicated that different groups of extremophilic fungi are present in the water and sediment samples of Lonar lake. A total of 38 species of fungi belonging to 18-different genera were recovered. Out of 104 isolates 32 showed ≤ 97% sequences similarity, which were morphologically different and could be potential novel isolates of extremophilic fungi. However, out of 104 isolates only 14 showed the extracellular phenol oxidase production potentials at alkaline pH. Curvularia sp. strain MEF018 showed highest phenol oxidase production at alkaline condition and had low sequence similarity with previously characterized species (96% with Curvularia pseudorobusta). Taxonomic characterization (morphological and physiological) and multi locus sequence analysis (MLSA) using combined alignment of ITS-LSU-gpd of strain MEF018 showed that it is a novel species of the genus Curvularia and hence proposed as Curvularia lonarensis sp. nov.
topic phenol oxidase
Fungal diversity
Curvularia
extremophilic fungi
Lonar- lake
Soda-lake
url http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01847/full
work_keys_str_mv AT rahulsharma diversityanddistributionofphenoloxidaseproducingfungifromsodalakeanddescriptionofcurvularialonarensisspnov
AT omprakash diversityanddistributionofphenoloxidaseproducingfungifromsodalakeanddescriptionofcurvularialonarensisspnov
AT maheshssonawane diversityanddistributionofphenoloxidaseproducingfungifromsodalakeanddescriptionofcurvularialonarensisspnov
AT yogeshnimonkar diversityanddistributionofphenoloxidaseproducingfungifromsodalakeanddescriptionofcurvularialonarensisspnov
AT priyankagollelu diversityanddistributionofphenoloxidaseproducingfungifromsodalakeanddescriptionofcurvularialonarensisspnov
AT rohitsharma diversityanddistributionofphenoloxidaseproducingfungifromsodalakeanddescriptionofcurvularialonarensisspnov
_version_ 1725658957415972864