Nutrient-Dependent Trade-Offs between Ribosomes and Division Protein Synthesis Control Bacterial Cell Size and Growth

Summary: Cell size control emerges from a regulated balance between the rates of cell growth and division. In bacteria, simple quantitative laws connect cellular growth rate to ribosome abundance. However, it remains poorly understood how translation regulates bacterial cell size and shape under gro...

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Main Authors:	Diana Serbanescu, Nikola Ojkic, Shiladitya Banerjee
Format:	Article
Language:	English
Published:	Elsevier 2020-09-01
Series:	Cell Reports
Subjects:	whole-cell model bacterial growth control cell size regulation translation antibiotics cell shape
Online Access:	http://www.sciencedirect.com/science/article/pii/S2211124720311724

id	doaj-344c6ea999c14da9bb4dd3f8a6e3d618
record_format	Article
spelling	doaj-344c6ea999c14da9bb4dd3f8a6e3d6182020-11-25T02:02:17ZengElsevierCell Reports2211-12472020-09-013212108183Nutrient-Dependent Trade-Offs between Ribosomes and Division Protein Synthesis Control Bacterial Cell Size and GrowthDiana Serbanescu0Nikola Ojkic1Shiladitya Banerjee2Department of Physics and Astronomy, University College London, London WC1E 6BT, UK; Institute for the Physics of Living Systems, University College London, London WC1E 6BT, UKDepartment of Physics and Astronomy, University College London, London WC1E 6BT, UK; Institute for the Physics of Living Systems, University College London, London WC1E 6BT, UKDepartment of Physics, Carnegie Mellon University, Pittsburgh, PA 15213, USA; Corresponding authorSummary: Cell size control emerges from a regulated balance between the rates of cell growth and division. In bacteria, simple quantitative laws connect cellular growth rate to ribosome abundance. However, it remains poorly understood how translation regulates bacterial cell size and shape under growth perturbations. Here, we develop a whole-cell model for growth dynamics of rod-shaped bacteria that links ribosomal abundance with cell geometry, division control, and the extracellular environment. Our study reveals that cell size maintenance under nutrient perturbations requires a balanced trade-off between ribosomes and division protein synthesis. Deviations from this trade-off relationship are predicted under translation inhibition, leading to distinct modes of cell morphological changes, in agreement with single-cell experimental data on Escherichia coli. Furthermore, by calibrating our model with experimental data, we predict how combinations of nutrient-, translational-, and shape perturbations can be chosen to optimize bacterial growth fitness and antibiotic resistance.http://www.sciencedirect.com/science/article/pii/S2211124720311724whole-cell modelbacterial growth controlcell size regulationtranslationantibioticscell shape
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Diana Serbanescu Nikola Ojkic Shiladitya Banerjee
spellingShingle	Diana Serbanescu Nikola Ojkic Shiladitya Banerjee Nutrient-Dependent Trade-Offs between Ribosomes and Division Protein Synthesis Control Bacterial Cell Size and Growth Cell Reports whole-cell model bacterial growth control cell size regulation translation antibiotics cell shape
author_facet	Diana Serbanescu Nikola Ojkic Shiladitya Banerjee
author_sort	Diana Serbanescu
title	Nutrient-Dependent Trade-Offs between Ribosomes and Division Protein Synthesis Control Bacterial Cell Size and Growth
title_short	Nutrient-Dependent Trade-Offs between Ribosomes and Division Protein Synthesis Control Bacterial Cell Size and Growth
title_full	Nutrient-Dependent Trade-Offs between Ribosomes and Division Protein Synthesis Control Bacterial Cell Size and Growth
title_fullStr	Nutrient-Dependent Trade-Offs between Ribosomes and Division Protein Synthesis Control Bacterial Cell Size and Growth
title_full_unstemmed	Nutrient-Dependent Trade-Offs between Ribosomes and Division Protein Synthesis Control Bacterial Cell Size and Growth
title_sort	nutrient-dependent trade-offs between ribosomes and division protein synthesis control bacterial cell size and growth
publisher	Elsevier
series	Cell Reports
issn	2211-1247
publishDate	2020-09-01
description	Summary: Cell size control emerges from a regulated balance between the rates of cell growth and division. In bacteria, simple quantitative laws connect cellular growth rate to ribosome abundance. However, it remains poorly understood how translation regulates bacterial cell size and shape under growth perturbations. Here, we develop a whole-cell model for growth dynamics of rod-shaped bacteria that links ribosomal abundance with cell geometry, division control, and the extracellular environment. Our study reveals that cell size maintenance under nutrient perturbations requires a balanced trade-off between ribosomes and division protein synthesis. Deviations from this trade-off relationship are predicted under translation inhibition, leading to distinct modes of cell morphological changes, in agreement with single-cell experimental data on Escherichia coli. Furthermore, by calibrating our model with experimental data, we predict how combinations of nutrient-, translational-, and shape perturbations can be chosen to optimize bacterial growth fitness and antibiotic resistance.
topic	whole-cell model bacterial growth control cell size regulation translation antibiotics cell shape
url	http://www.sciencedirect.com/science/article/pii/S2211124720311724
work_keys_str_mv	AT dianaserbanescu nutrientdependenttradeoffsbetweenribosomesanddivisionproteinsynthesiscontrolbacterialcellsizeandgrowth AT nikolaojkic nutrientdependenttradeoffsbetweenribosomesanddivisionproteinsynthesiscontrolbacterialcellsizeandgrowth AT shiladityabanerjee nutrientdependenttradeoffsbetweenribosomesanddivisionproteinsynthesiscontrolbacterialcellsizeandgrowth
_version_	1724953948515729408

Nutrient-Dependent Trade-Offs between Ribosomes and Division Protein Synthesis Control Bacterial Cell Size and Growth

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