Generation and validation of APOE knockout human iPSC-derived cerebral organoids

Generation and validation of APOE knockout human iPSC-derived cerebral organoids

Summary: Apolipoprotein E (apoE) is a major lipid carrier in the brain and closely associated with the pathogenesis of Alzheimer's disease (AD). Here, we describe a protocol for efficient knockout of APOE in human induced pluripotent stem cells (iPSCs) using the CRISPR-Cas9 system. We obtain ho...

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Bibliographic Details
Main Authors: Yuka A. Martens, Siming Xu, Richard Tait, Gary Li, Xinping C. Zhao, Wenyan Lu, Chia-Chen Liu, Takahisa Kanekiyo, Guojun Bu, Jing Zhao
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
CRISPR
Neuroscience
Stem Cells
Organoids
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721002781
Description
Summary:Summary: Apolipoprotein E (apoE) is a major lipid carrier in the brain and closely associated with the pathogenesis of Alzheimer's disease (AD). Here, we describe a protocol for efficient knockout of APOE in human induced pluripotent stem cells (iPSCs) using the CRISPR-Cas9 system. We obtain homozygous APOE knockout (APOE-/-) iPSCs and further validate the deficiency of apoE in iPSC-derived cerebral organoids. APOE-/- cerebral organoids can serve as a useful tool to study apoE functions and apoE-related pathogenic mechanisms in AD.For complete details on the use and execution of this protocol, please refer to Zhao et al. (2020).
ISSN:2666-1667

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