Generation of a homozygous ALX1 knockout human embryonic stem cell line (WAe001-A-060) by a CRISPR/Cas9 system

Generation of a homozygous ALX1 knockout human embryonic stem cell line (WAe001-A-060) by a CRISPR/Cas9 system

Human ALX1 gene (ALX Homeobox 1) is a protein coding gene and gene ontology annotations related to this gene include DNA-binding transcription factor activity and protein heterdimerization activity. It is necessary for survival of forebrain mesenchyme and may be involved in development of cervix. Ho...

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Main Authors: Xiuqing Xiao, Yashuang Chen, Lisheng Peng, Tian Zhang
Format: Article
Language:English
Published: Elsevier 2021-05-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506121001550
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spelling doaj-3444f47f9d4f4be28b1fea89ed31e2242021-05-30T04:41:28ZengElsevierStem Cell Research1873-50612021-05-0153102309Generation of a homozygous ALX1 knockout human embryonic stem cell line (WAe001-A-060) by a CRISPR/Cas9 systemXiuqing Xiao0Yashuang Chen1Lisheng Peng2Tian Zhang3Department of Neurology, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, ChinaDepartment of Neurology, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, ChinaDepartment of Neurology, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China; Corresponding authors.Medical Research Center, People's Hospital of Longhua, Shenzhen 518109, China; Corresponding authors.Human ALX1 gene (ALX Homeobox 1) is a protein coding gene and gene ontology annotations related to this gene include DNA-binding transcription factor activity and protein heterdimerization activity. It is necessary for survival of forebrain mesenchyme and may be involved in development of cervix. However, the function of the gene has yet to be determined in humans. Here we generated an ALX1 homozygous human embryonic stem cell line (WAe001-A-060) by a CRISPR/Cas9 system. The WAe001-A-060 has a normal undifferentiated morphology and karyotype, pluripotency and three germ layers differentiation potential in vivo.http://www.sciencedirect.com/science/article/pii/S1873506121001550
collection DOAJ
language English
format Article
sources DOAJ
author Xiuqing Xiao
Yashuang Chen
Lisheng Peng
Tian Zhang
spellingShingle Xiuqing Xiao
Yashuang Chen
Lisheng Peng
Tian Zhang
Generation of a homozygous ALX1 knockout human embryonic stem cell line (WAe001-A-060) by a CRISPR/Cas9 system
Stem Cell Research
author_facet Xiuqing Xiao
Yashuang Chen
Lisheng Peng
Tian Zhang
author_sort Xiuqing Xiao
title Generation of a homozygous ALX1 knockout human embryonic stem cell line (WAe001-A-060) by a CRISPR/Cas9 system
title_short Generation of a homozygous ALX1 knockout human embryonic stem cell line (WAe001-A-060) by a CRISPR/Cas9 system
title_full Generation of a homozygous ALX1 knockout human embryonic stem cell line (WAe001-A-060) by a CRISPR/Cas9 system
title_fullStr Generation of a homozygous ALX1 knockout human embryonic stem cell line (WAe001-A-060) by a CRISPR/Cas9 system
title_full_unstemmed Generation of a homozygous ALX1 knockout human embryonic stem cell line (WAe001-A-060) by a CRISPR/Cas9 system
title_sort generation of a homozygous alx1 knockout human embryonic stem cell line (wae001-a-060) by a crispr/cas9 system
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2021-05-01
description Human ALX1 gene (ALX Homeobox 1) is a protein coding gene and gene ontology annotations related to this gene include DNA-binding transcription factor activity and protein heterdimerization activity. It is necessary for survival of forebrain mesenchyme and may be involved in development of cervix. However, the function of the gene has yet to be determined in humans. Here we generated an ALX1 homozygous human embryonic stem cell line (WAe001-A-060) by a CRISPR/Cas9 system. The WAe001-A-060 has a normal undifferentiated morphology and karyotype, pluripotency and three germ layers differentiation potential in vivo.
url http://www.sciencedirect.com/science/article/pii/S1873506121001550
work_keys_str_mv AT xiuqingxiao generationofahomozygousalx1knockouthumanembryonicstemcelllinewae001a060byacrisprcas9system
AT yashuangchen generationofahomozygousalx1knockouthumanembryonicstemcelllinewae001a060byacrisprcas9system
AT lishengpeng generationofahomozygousalx1knockouthumanembryonicstemcelllinewae001a060byacrisprcas9system
AT tianzhang generationofahomozygousalx1knockouthumanembryonicstemcelllinewae001a060byacrisprcas9system
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