EFFECT OF AERO-/ANAEROBIOSIS ON DECARBOXYLASE ACTIVITY OF SELECTED LACTIC ACID BACTERIA

• Main Authors: Stanislav Kráčmar, Vladimír Dráb, Tereza Podešvová, Eva Pollaková, Leona Buňková, František Buňka
• Format: Article
• Language: English
• Published: HACCP Consulting 2010-05-01
• Series: Potravinarstvo
• Subjects: Lactococcus, tyramine, ion-exchange chromatography, aero/anaerobiosis
• Online Access: http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/43

<p><span style="font-size: 10.0pt; font-family: "Times New Roman","serif"; mso-fareast-font-family: "Times New Roman"; mso-ansi-language: SK; mso-fareast-language: SK; mso-bidi-language: AR-SA; mso-bidi-font-weight: bold;">Biogenic amines are undesirable compounds produced in foods mainly through bacterial decarboxylase activity. The aim of this study was to investigate some environmental conditions (particularly aero/anaerobiosis, sodium chloride concentration (0&ndash;2% w/w), and amount of lactose (0&ndash;1% w/w)) on the activity of tyrosine decarboxylase enzymes of selected six technological important Lactococcus lactis strains. The levels of parameters tested were chosen according to real situation in fermented dairy products technology (especially cheese-making). Tyramine was determined by the ion-exchange chromatography with post-column ninhydrine derivatization and spectrophotometric detection. Tyrosine decarboxylation occurred during the active growth phase. Under the model conditions used, oxygen availability had influence on tyramine production, anaerobiosis seemed to favour the enzyme activity because all L. lactis strains produced higher tyramine amount.</span></p><p>&nbsp;</p><p><span style="font-size: 10.0pt; font-family: "Times New Roman","serif"; mso-fareast-font-family: "Times New Roman"; mso-ansi-language: SK; mso-fareast-language: SK; mso-bidi-language: AR-SA; mso-bidi-font-weight: bold;"><span style="font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 12px;"><strong>doi:10.5219/43</strong></span></span></p>