Application of Scharer's quantitative method for the determination of residual alkaline phosphatase activity in standard Minas

• Main Authors: C.F. Soares, L.M. Fonseca, M.O. Leite, M.C.P.P. Oliveira
• Format: Article
• Language: English
• Published: Universidade Federal de Minas Gerais 2013-08-01
• Series: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
• Subjects: fosfatase alcalina; queijo minas padrão; método modificado de Scharer; espectrofotometria
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000400039&lng=en&tlng=en

Milk pasteurization is a critical issue in the dairy industry, and failures in this process can affect final product safety. Scharer's enzymatic method is still traditionally used to verify pasteurization efficiency compliance, and it is based on screening for residual alkaline phosphatase in milk. Although several methods are used to quantify enzymatic activity to assess milk pasteurization efficiency, there is a small amount of published data regarding the use of these methods to quantify alkaline phosphatase in cheese. In this study, the Scharer's modified method was used to determine the levels of residual alkaline phosphatase in standard minas cheese, before and after 20 days of ripening. The cheeses were made using raw or pasteurized milk with the addition of different concentrations of raw milk (0; 0.05%; 0.10%; 0.20%; and 0.50%). In the fresh cheese samples, the method showed a sensitivity of only 0.50% with the addition of raw milk to the pasteurized milk used to make cheese. In addition, levels of up 0.20% of raw milk in pasteurized milk, the concentrations of phenol was inferior to 1μg phenol/g of dairy product which is the preconized indicator value for adequate pasteurization.