Testis single-cell RNA-seq reveals the dynamics of de novo gene transcription and germline mutational bias in Drosophila
The testis is a peculiar tissue in many respects. It shows patterns of rapid gene evolution and provides a hotspot for the origination of genetic novelties such as de novo genes, duplications and mutations. To investigate the expression patterns of genetic novelties across cell types, we performed s...
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eLife Sciences Publications Ltd
2019-08-01
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| Online Access: | https://elifesciences.org/articles/47138 |
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doaj-33c9007196ad49e69fff76df0a40d6752021-05-05T17:50:53ZengeLife Sciences Publications LtdeLife2050-084X2019-08-01810.7554/eLife.47138Testis single-cell RNA-seq reveals the dynamics of de novo gene transcription and germline mutational bias in DrosophilaEvan Witt0https://orcid.org/0000-0003-2973-6946Sigi Benjamin1https://orcid.org/0000-0002-6411-5339Nicolas Svetec2https://orcid.org/0000-0001-9617-2752Li Zhao3https://orcid.org/0000-0001-6776-1996Laboratory of Evolutionary Genetics and Genomics, The Rockefeller University, New York, United StatesLaboratory of Evolutionary Genetics and Genomics, The Rockefeller University, New York, United StatesLaboratory of Evolutionary Genetics and Genomics, The Rockefeller University, New York, United StatesLaboratory of Evolutionary Genetics and Genomics, The Rockefeller University, New York, United StatesThe testis is a peculiar tissue in many respects. It shows patterns of rapid gene evolution and provides a hotspot for the origination of genetic novelties such as de novo genes, duplications and mutations. To investigate the expression patterns of genetic novelties across cell types, we performed single-cell RNA-sequencing of adult Drosophila testis. We found that new genes were expressed in various cell types, the patterns of which may be influenced by their mode of origination. In particular, lineage-specific de novo genes are commonly expressed in early spermatocytes, while young duplicated genes are often bimodally expressed. Analysis of germline substitutions suggests that spermatogenesis is a highly reparative process, with the mutational load of germ cells decreasing as spermatogenesis progresses. By elucidating the distribution of genetic novelties across spermatogenesis, this study provides a deeper understanding of how the testis maintains its core reproductive function while being a hotbed of evolutionary innovation.https://elifesciences.org/articles/47138de novo genemutational loadsingle-cell sequencingexpression dynamicsDrosophilaspermatogenesis |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Evan Witt Sigi Benjamin Nicolas Svetec Li Zhao |
| spellingShingle |
Evan Witt Sigi Benjamin Nicolas Svetec Li Zhao Testis single-cell RNA-seq reveals the dynamics of de novo gene transcription and germline mutational bias in Drosophila eLife de novo gene mutational load single-cell sequencing expression dynamics Drosophila spermatogenesis |
| author_facet |
Evan Witt Sigi Benjamin Nicolas Svetec Li Zhao |
| author_sort |
Evan Witt |
| title |
Testis single-cell RNA-seq reveals the dynamics of de novo gene transcription and germline mutational bias in Drosophila |
| title_short |
Testis single-cell RNA-seq reveals the dynamics of de novo gene transcription and germline mutational bias in Drosophila |
| title_full |
Testis single-cell RNA-seq reveals the dynamics of de novo gene transcription and germline mutational bias in Drosophila |
| title_fullStr |
Testis single-cell RNA-seq reveals the dynamics of de novo gene transcription and germline mutational bias in Drosophila |
| title_full_unstemmed |
Testis single-cell RNA-seq reveals the dynamics of de novo gene transcription and germline mutational bias in Drosophila |
| title_sort |
testis single-cell rna-seq reveals the dynamics of de novo gene transcription and germline mutational bias in drosophila |
| publisher |
eLife Sciences Publications Ltd |
| series |
eLife |
| issn |
2050-084X |
| publishDate |
2019-08-01 |
| description |
The testis is a peculiar tissue in many respects. It shows patterns of rapid gene evolution and provides a hotspot for the origination of genetic novelties such as de novo genes, duplications and mutations. To investigate the expression patterns of genetic novelties across cell types, we performed single-cell RNA-sequencing of adult Drosophila testis. We found that new genes were expressed in various cell types, the patterns of which may be influenced by their mode of origination. In particular, lineage-specific de novo genes are commonly expressed in early spermatocytes, while young duplicated genes are often bimodally expressed. Analysis of germline substitutions suggests that spermatogenesis is a highly reparative process, with the mutational load of germ cells decreasing as spermatogenesis progresses. By elucidating the distribution of genetic novelties across spermatogenesis, this study provides a deeper understanding of how the testis maintains its core reproductive function while being a hotbed of evolutionary innovation. |
| topic |
de novo gene mutational load single-cell sequencing expression dynamics Drosophila spermatogenesis |
| url |
https://elifesciences.org/articles/47138 |
| work_keys_str_mv |
AT evanwitt testissinglecellrnaseqrevealsthedynamicsofdenovogenetranscriptionandgermlinemutationalbiasindrosophila AT sigibenjamin testissinglecellrnaseqrevealsthedynamicsofdenovogenetranscriptionandgermlinemutationalbiasindrosophila AT nicolassvetec testissinglecellrnaseqrevealsthedynamicsofdenovogenetranscriptionandgermlinemutationalbiasindrosophila AT lizhao testissinglecellrnaseqrevealsthedynamicsofdenovogenetranscriptionandgermlinemutationalbiasindrosophila |
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1721458960366567424 |