THE EFFECTIVENESS OF IMMUNOVAC VP-4 FOR IMMUNOLOGICAL PARAMETERS IN FREQUENTLY AND LONG-TERM ILL CHILDREN

• Main Authors: E. P. Foshina, T. A. Serova, I. B. Bisheva, O. V. Slatinova
• Format: Article
• Language: Russian
• Published: Central Research Institute for Epidemiology 2019-08-01
• Series: Журнал микробиологии, эпидемиологии и иммунобиологии
• Subjects: frequently and long-term ill children; specific antibodies; iga-isotype antibodies; bacterial immunomodulators; mucosal immunity
• Online Access: https://microbiol.elpub.ru/jour/article/view/402
• ISSN: 0372-9311; 2686-7613

Aim. To study the level of specific antibodies of different isotypes to the antigens of Staphylococcus aureus and Klebsiella рneumoniae in the serum, saliva and nazal secret and the concentration of IgA,sIgA,IgG in saliva from frequently and long-term ill children in nasal-oral administration of Immunovac VP-4. Materials and methods. Specific antibodies to S. aureus and K.pneumoniae, contained in saliva, nasal, and serum were determined by the method of enzyme immunoassay. Concentrations of immunoglobulins of classes G, A and sА in saliva were determined by radial immunodiffusion using a commercial kit produced by the NPC «Medical immunology». Results. The high level of specific antibodies contained in the serum and nasal secretions, the level of antibodies in saliva is negligible. The serum is dominated by IgG-isotype antibodies, saliva and nasal secret — antibodies of IgA-isotope. After the introduction of Immunovac VP-4 there was a statistically significant increase in the level of specific antibacterial antibodies in serum, saliva and nasal secret, and increasing levels of IgG and sIgA in saliva. Conclusion. Obtained data demonstratet that the nasal-oral scheme of administration of Immunovac VP-4 frequently and long-term ill children allowed to increase the level of specific antibodies in serum, saliva and nasal secret to bacterial antigens that are part of the vaccine and to normalize the local synthesis of IgG and sIgA, which play a major role in the protection of the respiratory tract and mucous membranes of the upper respiratory tract.