Whole genome analysis of CRISPR Cas9 sgRNA off-target homologies via an efficient computational algorithm

Whole genome analysis of CRISPR Cas9 sgRNA off-target homologies via an efficient computational algorithm

Abstract Background The beauty and power of the genome editing mechanism, CRISPR Cas9 endonuclease system, lies in the fact that it is RNA-programmable such that Cas9 can be guided to any genomic loci complementary to a 20-nt RNA, single guide RNA (sgRNA), to cleave double stranded DNA, allowing the...

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Bibliographic Details
Main Authors: Hong Zhou, Michael Zhou, Daisy Li, Joseph Manthey, Ekaterina Lioutikova, Hong Wang, Xiao Zeng
Format: Article
Language:English
Published: BMC 2017-11-01
Series:BMC Genomics
Subjects:
sgRNA
Off-target homology
Crispr
Cas9
Computational algorithm
Genome wide
Online Access:http://link.springer.com/article/10.1186/s12864-017-4225-1

Internet

http://link.springer.com/article/10.1186/s12864-017-4225-1

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