A simple method for HPLC retention time prediction: linear calibration using two reference substances

A simple method for HPLC retention time prediction: linear calibration using two reference substances

Abstract Background Analysis of related substances in pharmaceutical chemicals and multi-components in traditional Chinese medicines needs bulk of reference substances to identify the chromatographic peaks accurately. But the reference substances are costly. Thus, the relative retention (RR) method...

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Bibliographic Details
Main Authors: Lei Sun, Hong-yu Jin, Run-tao Tian, Ming-juan Wang, Li-na Liu, Liu-ping Ye, Tian-tian Zuo, Shuang-cheng Ma
Format: Article
Language:English
Published: BMC 2017-06-01
Series:Chinese Medicine
Subjects:
RP-HPLC
Retention time
Relative retention
Linear calibration using two reference substances
Multi-component analysis
Traditional Chinese medicines
Online Access:http://link.springer.com/article/10.1186/s13020-017-0137-x

Internet

http://link.springer.com/article/10.1186/s13020-017-0137-x

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