Genome-wide identification of WD40 superfamily genes and prediction of WD40 gene of flavonoid-related genes in Ginkgo biloba

• Main Authors: Jiarui ZHENG, Yongling LIAO, Feng XU, Xian ZHOU, Jiabao YE, Mingyue FU, Xiaomeng LIU, Zhengyan CAO, Weiwei ZHANG
• Format: Article
• Language: English
• Published: AcademicPres 2021-06-01
• Series: Notulae Botanicae Horti Agrobotanici Cluj-Napoca
• Subjects: flavonoid; genome; Ginkgo biloba; superfamily genes; WD40
• Online Access: https://www.notulaebotanicae.ro/index.php/nbha/article/view/12086
• ISSN: 0255-965X; 1842-4309

The WD40 transcription factor family is a superfamily found in eukaryotes and implicated in regulating growth and development. In this study, 167 WD40 family genes are identified in the Ginkgo biloba genome. They are divided into 5 clusters and 16 subfamilies based on the difference analysis of a phylogenetic tree and domain structures. The distribution of WD40 genes in chromosomes, gene structures, and motifs is analyzed. Promoter analysis shows that five GbWD40 gene promoters contain the MYB binding site participating in the regulation of flavonoid metabolism, suggesting that these five genes may participate in the regulation of flavonoid synthesis in G. biloba. The correlation analysis is carried out based on FPKM value of WD40 gene and flavonoid content in 8 tissues of G. biloba. Six GbWD40 genes that may participate in flavonoid metabolism are screened. The biological functions of the WD40 family genes in G. biloba are systematically analyzed, providing a foundation for further elucidating their regulatory mechanisms. A number of WD40 candidate genes involved in the biosynthesis and metabolism of G. biloba also predicted. This study presents an important basis and direction for conducting further research on the regulatory network of flavonoid synthesis and metabolism.