Development of an efficient protocol for production of healthy sugarcane seed cane through Meristem culture

Healthy seeds are a prerequisite for improving the productivity of sugarcane crop. The best way to ensure the production of disease-free sugarcane seeds is via a technique called ‘Meristem Culture’ (Tissue Culture). An efficient protocol for meristem culture of sugarcane has been developed in this s...

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Main Author: Shubhangi Salokhe
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:Journal of Agriculture and Food Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666154321000284
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spelling doaj-331f4bb6ba344c6286f8c3c525d5bfcb2021-05-18T04:11:30ZengElsevierJournal of Agriculture and Food Research2666-15432021-06-014100126Development of an efficient protocol for production of healthy sugarcane seed cane through Meristem cultureShubhangi Salokhe0Symbiosis Foundation, Pune, IndiaHealthy seeds are a prerequisite for improving the productivity of sugarcane crop. The best way to ensure the production of disease-free sugarcane seeds is via a technique called ‘Meristem Culture’ (Tissue Culture). An efficient protocol for meristem culture of sugarcane has been developed in this study. The potting mix for the survival of meristem cultured plantlets in a greenhouse was standardized, and agronomy for growing tissue culture plantlets in the field was developed. Multiple shoots were observed on Murashige and Skoog's (MS) medium supplemented with BAP 0.2 ​mg/l and Kinetin 0.1 ​mg/l. Best rooting was observed on MS media supplemented with IAA 1 ​mg/l and NAA 1 ​mg/l. Soil mixture containing [Soil ​+ ​Vermiculite ​+ ​Sand in 4: 1: 1 proportion (by volume)] ​+ ​Vermicompost @ 25% by volume of medium ​+ ​NPK is best suited for hardening tissue cultured plants in a greenhouse. Two feet distance between 2 plants and 3 feet distance between 2 rows was found as an appropriate method for planting tissue cultured plantlets in the field. Setts obtained from tissue cultured plants planted at 15 ​cm spacing gave higher yield than the other spacing. Setts obtained from micro-propagated plants (M) gave the highest yield as compared to setts from conventional sugarcane raising. The protocol developed in this study, shows potential for development of sugarcane meristem cultured plantlets and use of these plants for planting in field to obtain healthy sugarcane seed.http://www.sciencedirect.com/science/article/pii/S2666154321000284BiotechnologyProtocolSugarcaneSeedTissue culture
collection DOAJ
language English
format Article
sources DOAJ
author Shubhangi Salokhe
spellingShingle Shubhangi Salokhe
Development of an efficient protocol for production of healthy sugarcane seed cane through Meristem culture
Journal of Agriculture and Food Research
Biotechnology
Protocol
Sugarcane
Seed
Tissue culture
author_facet Shubhangi Salokhe
author_sort Shubhangi Salokhe
title Development of an efficient protocol for production of healthy sugarcane seed cane through Meristem culture
title_short Development of an efficient protocol for production of healthy sugarcane seed cane through Meristem culture
title_full Development of an efficient protocol for production of healthy sugarcane seed cane through Meristem culture
title_fullStr Development of an efficient protocol for production of healthy sugarcane seed cane through Meristem culture
title_full_unstemmed Development of an efficient protocol for production of healthy sugarcane seed cane through Meristem culture
title_sort development of an efficient protocol for production of healthy sugarcane seed cane through meristem culture
publisher Elsevier
series Journal of Agriculture and Food Research
issn 2666-1543
publishDate 2021-06-01
description Healthy seeds are a prerequisite for improving the productivity of sugarcane crop. The best way to ensure the production of disease-free sugarcane seeds is via a technique called ‘Meristem Culture’ (Tissue Culture). An efficient protocol for meristem culture of sugarcane has been developed in this study. The potting mix for the survival of meristem cultured plantlets in a greenhouse was standardized, and agronomy for growing tissue culture plantlets in the field was developed. Multiple shoots were observed on Murashige and Skoog's (MS) medium supplemented with BAP 0.2 ​mg/l and Kinetin 0.1 ​mg/l. Best rooting was observed on MS media supplemented with IAA 1 ​mg/l and NAA 1 ​mg/l. Soil mixture containing [Soil ​+ ​Vermiculite ​+ ​Sand in 4: 1: 1 proportion (by volume)] ​+ ​Vermicompost @ 25% by volume of medium ​+ ​NPK is best suited for hardening tissue cultured plants in a greenhouse. Two feet distance between 2 plants and 3 feet distance between 2 rows was found as an appropriate method for planting tissue cultured plantlets in the field. Setts obtained from tissue cultured plants planted at 15 ​cm spacing gave higher yield than the other spacing. Setts obtained from micro-propagated plants (M) gave the highest yield as compared to setts from conventional sugarcane raising. The protocol developed in this study, shows potential for development of sugarcane meristem cultured plantlets and use of these plants for planting in field to obtain healthy sugarcane seed.
topic Biotechnology
Protocol
Sugarcane
Seed
Tissue culture
url http://www.sciencedirect.com/science/article/pii/S2666154321000284
work_keys_str_mv AT shubhangisalokhe developmentofanefficientprotocolforproductionofhealthysugarcaneseedcanethroughmeristemculture
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