Molecular Analysis of <em>Fomitiporia mediterranea</em> Isolates from Esca-Affected Grapevines in Southern Italy
Four Fomitiporia isolates representative of a collection of about 300 isolates from esca-affected grapevines in southern Italy (Apulia, Campania and Abruzzo) were examined by molecular methods. The DNA of each isolate was analysed by means of polymerase chain reaction (PCR amplification) using ITS5...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Firenze University Press
2004-08-01
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| Series: | Phytopathologia Mediterranea |
| Online Access: | https://oajournals.fupress.net/index.php/pm/article/view/5056 |
| Summary: | Four Fomitiporia isolates representative of a collection of about 300 isolates from esca-affected grapevines
in southern Italy (Apulia, Campania and Abruzzo) were examined by molecular methods. The DNA of each
isolate was analysed by means of polymerase chain reaction (PCR amplification) using ITS5 and ITS4 primers. An
amplification product of 740 bp was obtained from all isolates. The denaturated products had the same migration
pattern when analysed by single-strand conformation polymorphism. The PCR fragments that included the ribosomal
ITS1-5.8S-ITS2 region were sequenced. In order to ascertain the taxonomic identity of the isolates, the ITS
sequences were compared with those of Fomitiporia mediterranea, F. punctata and F. robusta deposited in the GenBank.
The ITS sequences of the isolates were uniform and homologous with those of the type culture of Fomitiporia
mediterranea M. Fischer, to which the southern Italian isolates were compared. |
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| ISSN: | 0031-9465 1593-2095 |