Post-correlation on-lamella cryo-CLEM reveals the membrane architecture of lamellar bodies

Post-correlation on-lamella cryo-CLEM reveals the membrane architecture of lamellar bodies

Using the post-correlation on-lamella cryo-CLEM workflow, Klein, Wimmer et al. show that lamellar bodies (LBs) are composed of membrane sheets frequently attached to the limiting membrane through T-junctions in ABCA3 overexpressing cells and in primary human small airway epithelial cells. This study...

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Main Authors: Steffen Klein, Benedikt H. Wimmer, Sophie L. Winter, Androniki Kolovou, Vibor Laketa, Petr Chlanda
Format: Article
Language:English
Published: Nature Publishing Group 2021-01-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-020-01567-z
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spelling doaj-32ea53411acf49449270aa8a27ac41732021-01-31T16:16:16ZengNature Publishing GroupCommunications Biology2399-36422021-01-014111210.1038/s42003-020-01567-zPost-correlation on-lamella cryo-CLEM reveals the membrane architecture of lamellar bodiesSteffen Klein0Benedikt H. Wimmer1Sophie L. Winter2Androniki Kolovou3Vibor Laketa4Petr Chlanda5Schaller Research Groups, Department of Infectious Diseases-Virology, Heidelberg UniversitySchaller Research Groups, Department of Infectious Diseases-Virology, Heidelberg UniversitySchaller Research Groups, Department of Infectious Diseases-Virology, Heidelberg UniversitySchaller Research Groups, Department of Infectious Diseases-Virology, Heidelberg UniversityInfectious Diseases Imaging Platform (IDIP), Department of Infectious Diseases, Heidelberg UniversitySchaller Research Groups, Department of Infectious Diseases-Virology, Heidelberg UniversityUsing the post-correlation on-lamella cryo-CLEM workflow, Klein, Wimmer et al. show that lamellar bodies (LBs) are composed of membrane sheets frequently attached to the limiting membrane through T-junctions in ABCA3 overexpressing cells and in primary human small airway epithelial cells. This study provides insights into LB biogenesis and membrane packing inside the LB.https://doi.org/10.1038/s42003-020-01567-z
collection DOAJ
language English
format Article
sources DOAJ
author Steffen Klein
Benedikt H. Wimmer
Sophie L. Winter
Androniki Kolovou
Vibor Laketa
Petr Chlanda
spellingShingle Steffen Klein
Benedikt H. Wimmer
Sophie L. Winter
Androniki Kolovou
Vibor Laketa
Petr Chlanda
Post-correlation on-lamella cryo-CLEM reveals the membrane architecture of lamellar bodies
Communications Biology
author_facet Steffen Klein
Benedikt H. Wimmer
Sophie L. Winter
Androniki Kolovou
Vibor Laketa
Petr Chlanda
author_sort Steffen Klein
title Post-correlation on-lamella cryo-CLEM reveals the membrane architecture of lamellar bodies
title_short Post-correlation on-lamella cryo-CLEM reveals the membrane architecture of lamellar bodies
title_full Post-correlation on-lamella cryo-CLEM reveals the membrane architecture of lamellar bodies
title_fullStr Post-correlation on-lamella cryo-CLEM reveals the membrane architecture of lamellar bodies
title_full_unstemmed Post-correlation on-lamella cryo-CLEM reveals the membrane architecture of lamellar bodies
title_sort post-correlation on-lamella cryo-clem reveals the membrane architecture of lamellar bodies
publisher Nature Publishing Group
series Communications Biology
issn 2399-3642
publishDate 2021-01-01
description Using the post-correlation on-lamella cryo-CLEM workflow, Klein, Wimmer et al. show that lamellar bodies (LBs) are composed of membrane sheets frequently attached to the limiting membrane through T-junctions in ABCA3 overexpressing cells and in primary human small airway epithelial cells. This study provides insights into LB biogenesis and membrane packing inside the LB.
url https://doi.org/10.1038/s42003-020-01567-z
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