Multiple types of human papillomavirus in cervical samples in women in Campo Grande, MS, Brazil

• Main Authors: Inês Aparecida Tozetti, Ilzia Doraci Lins Scapulatempo, Vicky Liege Kawski, Antonio Walter Ferreira, José Eduardo Levi
• Format: Article
• Language: English
• Published: Elsevier
• Series: Brazilian Journal of Infectious Diseases
• Subjects: Human papillomavirus; multiple types; nested PCR; TS-PCR
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000500001&lng=en&tlng=en

We analyzed 87 cervical samples from Campo Grande, Mato Grosso do Sul, with a PGMY/GP+ nested PCR system. Positive samples were typed using E7 type-specific primer pairs for HPV 6/11, 16, 18, 45 and 66. Eighteen samples (22%) were infected with HPV6/11, 18 samples (22%) with HPV66, 13 samples (15.9%) with HPV45, 8 samples (9.8%) with HPV18 and 7 samples (8.5%) with HPV16. Seventeen samples (20.7%) were infected by two HPV types, and five samples (6.1%) by three HPV types. We conclude that infection with multiple types is present at a high frequency in our population and that there is a relation between some types and cytological finds.