Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues

Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues

Super-resolution imaging of multiple target proteins in the same sample can provide important information of cellular nanostructure, but has not been routinely achieved. Here, the authors present a fully automated 3D STORM approach using a re-staining protocol to image 15 targets in single cells and...

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Main Authors: Maja Klevanski, Frank Herrmannsdoerfer, Steffen Sass, Varun Venkataramani, Mike Heilemann, Thomas Kuner
Format: Article
Language:English
Published: Nature Publishing Group 2020-03-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-15362-1
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spelling doaj-32a771440bc44ff9ab58e746e4a6a5a52021-05-11T08:51:09ZengNature Publishing GroupNature Communications2041-17232020-03-0111111110.1038/s41467-020-15362-1Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissuesMaja Klevanski0Frank Herrmannsdoerfer1Steffen Sass2Varun Venkataramani3Mike Heilemann4Thomas Kuner5Department of Functional Neuroanatomy, Institute for Anatomy and Cell Biology, Heidelberg UniversityDepartment of Functional Neuroanatomy, Institute for Anatomy and Cell Biology, Heidelberg UniversityDepartment of Functional Neuroanatomy, Institute for Anatomy and Cell Biology, Heidelberg UniversityDepartment of Functional Neuroanatomy, Institute for Anatomy and Cell Biology, Heidelberg UniversityDepartment of Functional Neuroanatomy, Institute for Anatomy and Cell Biology, Heidelberg UniversityDepartment of Functional Neuroanatomy, Institute for Anatomy and Cell Biology, Heidelberg UniversitySuper-resolution imaging of multiple target proteins in the same sample can provide important information of cellular nanostructure, but has not been routinely achieved. Here, the authors present a fully automated 3D STORM approach using a re-staining protocol to image 15 targets in single cells and 16 targets in neuronal tissue.https://doi.org/10.1038/s41467-020-15362-1
collection DOAJ
language English
format Article
sources DOAJ
author Maja Klevanski
Frank Herrmannsdoerfer
Steffen Sass
Varun Venkataramani
Mike Heilemann
Thomas Kuner
spellingShingle Maja Klevanski
Frank Herrmannsdoerfer
Steffen Sass
Varun Venkataramani
Mike Heilemann
Thomas Kuner
Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues
Nature Communications
author_facet Maja Klevanski
Frank Herrmannsdoerfer
Steffen Sass
Varun Venkataramani
Mike Heilemann
Thomas Kuner
author_sort Maja Klevanski
title Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues
title_short Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues
title_full Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues
title_fullStr Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues
title_full_unstemmed Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues
title_sort automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2020-03-01
description Super-resolution imaging of multiple target proteins in the same sample can provide important information of cellular nanostructure, but has not been routinely achieved. Here, the authors present a fully automated 3D STORM approach using a re-staining protocol to image 15 targets in single cells and 16 targets in neuronal tissue.
url https://doi.org/10.1038/s41467-020-15362-1
work_keys_str_mv AT majaklevanski automatedhighlymultiplexedsuperresolutionimagingofproteinnanoarchitectureincellsandtissues
AT frankherrmannsdoerfer automatedhighlymultiplexedsuperresolutionimagingofproteinnanoarchitectureincellsandtissues
AT steffensass automatedhighlymultiplexedsuperresolutionimagingofproteinnanoarchitectureincellsandtissues
AT varunvenkataramani automatedhighlymultiplexedsuperresolutionimagingofproteinnanoarchitectureincellsandtissues
AT mikeheilemann automatedhighlymultiplexedsuperresolutionimagingofproteinnanoarchitectureincellsandtissues
AT thomaskuner automatedhighlymultiplexedsuperresolutionimagingofproteinnanoarchitectureincellsandtissues
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