The correlation of serum MIF and IGFBPs levels with the cancer cell viability in the focus of patients with early lung cancer

• Main Authors: Hui-Guang Li1, Gui-Quan Gao2
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2017-04-01
• Series: Journal of Hainan Medical University
• Subjects: Non-small cell lung cancer Macrophage inhibitory cytokine Insulin-like growth factor binding protein Silent information regulator-1 Epithelial-mesenchymal transition
• Online Access: http://www.hnykdxxb.com/PDF/201707/35.pdf

Objective: To study the correlation of serum Macrophage inhibitory factor (MIF) and insulinlike growth factor binding proteins (IFGBPs) levels with the cancer cell viability in the focus of patients with early lung cancer. Methods: 38 patients with early non-small cell lung cancer who received surgical resection in our hospital between May 2013 and August 2016 were selected as the lung cancer group, and 60 healthy volunteers who received physical examination during the same period were selected as the control group. The serum was collected from two groups of subjects to determine the MIF and IGFBPs levels, and the lung cancer tissue and the tissue adjacent to carcinoma were collected from the lung cancer group to determine the mRNA expression of SIRT-1-related molecules. Results: Serum MIF level of lung cancer group was significantly higher than that of control group (P＜0.05), IGFBP-3 level was significantly lower than that of control group (P＜0.05), and the IGFBP-2 and IGFBP-5 levels were not significantly different from those of control group; SIRT-1, Vimentin and N-cadherin mRNA expression in lung cancer tissue were significantly higher than those in the tissue adjacent to carcinoma (P＜0.05) while ZO-1 and E-cadherin mRNA expression were significantly lower than those in the tissue adjacent to carcinoma (P＜0.05); SIRT-1, Vimentin and N-cadherin mRNA expression were higher while ZO-1 and E-cadherin mRNA expression were lower in focus tissue of lung cancer patients with high MIF levels and those with low IGFBP-3 levels. Conclusion: Serum MIF level increases abnormally while IGFBP-3 level decreases abnormally in patients with early lung cancer, and the change of MIF and IGFBP-3 levels is correlated with the change of cancer cell growth process mediated by SIRT-1.